Alpha-amylase 1 ELISA 试剂盒
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- 抗原 See all Alpha-amylase 1 (AMY1) ELISA试剂盒
- Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes natural and recombinantRatAMY1. No significant cross-reactivity or interference between RatAMY1 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatAMY1 and all the analogues.
- 灵敏度
- 0.94 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
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Amylase 1, Salivary (AMY1) ELISA Kit
AMY1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Amylase 1, Salivary (AMY1) ELISA KitAMY1 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Amylase 1, Salivary (AMY1) ELISA KitAMY1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Amylase 1, Salivary (AMY1) ELISA KitAMY1 适用: 人 Colorimetric Sandwich ELISA 0.469 μg/mL - 30 μg/mL Plasma, Serum, Tissue Homogenate
Amylase 1, Salivary (AMY1) ELISA KitAMY1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Amylase 1, Salivary (AMY1) ELISA KitAMY1 适用: 人 Colorimetric Sandwich ELISA 7.8 IU/mL - 500 IU/mL Saliva, Serum, Tissue Homogenate
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- 说明
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Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - 样本量
- 100 µL
- 板类型
- Pre-coated
- 实验流程
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of . Then make serial dilutions as needed. The recommended dilution gradient is as follows: , , , , , , , 0 . Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the working solution to the first tube and mix up to produce a working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 限制
- 仅限研究用
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- 注意事项
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
-
- 抗原 See all Alpha-amylase 1 (AMY1) ELISA试剂盒
- Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))
- 别名
- Amylase Alpha 1, Salivary (AMY1) (AMY1 产品)
- 别名
- AMY1 ELISA Kit, AMY2 ELISA Kit, AMY2B ELISA Kit, PA ELISA Kit, Amy-1 ELISA Kit, Amy1a ELISA Kit, C030014B17Rik ELISA Kit, Amy1 ELISA Kit, amylase, alpha 1C (salivary) ELISA Kit, amylase, alpha 2A (pancreatic) ELISA Kit, amylase 1, salivary ELISA Kit, amylase, alpha 1A ELISA Kit, amylase, alpha 1A (salivary) ELISA Kit, AMY1C ELISA Kit, AMY2A ELISA Kit, Amy1 ELISA Kit, Amy1a ELISA Kit, AMY1A ELISA Kit
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