SHC1 ELISA 试剂盒
SHC1
适用: 人, 小鼠, 大鼠
pTyr427
Colorimetric
Sandwich ELISA
Cell Culture Lysate
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- 抗原 See all SHC1 ELISA试剂盒
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
- 抗原表位
- pTyr427
- 适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phospho-SHC (Tyr427) ELISA Kit. This assay semi-quantitatively measures SHC phosphorylated at Tyrosine-427 in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human, Mouse and Rat SHC phosphorylated at site Tyrosine-427.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product SHC1 ELISA Kit
-
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) ELISA Kit
SHC1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) ELISA KitSHC1 适用: 人, 小鼠, 大鼠 pTyr427 Colorimetric Sandwich ELISA Cell Culture Lysate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all SHC1 ELISA试剂盒
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
- 别名
- SHC (SHC1 产品)
- 别名
- SHC1 ELISA Kit, SHC ELISA Kit, SHCA ELISA Kit, P66shc ELISA Kit, si:dkey-246i14.4 ELISA Kit, wu:fc10h03 ELISA Kit, shc ELISA Kit, p66shc ELISA Kit, Shc ELISA Kit, ShcA ELISA Kit, p66 ELISA Kit, SHC adaptor protein 1 ELISA Kit, Shc1p ELISA Kit, squalene--hopene cyclase ELISA Kit, SHC (Src homology 2 domain containing) transforming protein 1 ELISA Kit, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog ELISA Kit, src homology 2 domain-containing transforming protein C1 ELISA Kit, SHC1 ELISA Kit, shc1 ELISA Kit, Shc1 ELISA Kit, shc1.L ELISA Kit
- 基因ID
- 6464
- UniProt
- P29353, P98083, O70143
- 途径
- RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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