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EIF2B1 ELISA 试剂盒

EIF2B1 适用: 人 pSer52 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
产品编号 ABIN4886044
发货至: 中国
  • 抗原 See all EIF2B1 ELISA试剂盒
    EIF2B1 (Eukaryotic Translation Initiation Factor 2B, Subunit 1 Alpha, 26kDa (EIF2B1))
    抗原表位
    pSer52, total
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Phospho-EIF2A (S52) and Total EIF2A ELISA Kit. This assay semi-quantitatively measures phophorylated EIF2A (Ser52) and Total EIF2A in lysate samples.
    样品类型
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    The antibody pair provided in this kit recognizes Human eIF-2A phosphorylated at site Serine-52 and Total eIF-2a.
    产品特性
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product EIF2B1 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all EIF2B1 ELISA试剂盒
    EIF2B1 (Eukaryotic Translation Initiation Factor 2B, Subunit 1 Alpha, 26kDa (EIF2B1))
    别名
    eIF-2a (EIF2B1 产品)
    别名
    DDBDRAFT_0219393 ELISA Kit, DDBDRAFT_0231375 ELISA Kit, DDB_0219393 ELISA Kit, DDB_0231375 ELISA Kit, 26kDa ELISA Kit, D5Ertd406e ELISA Kit, EIF2B ELISA Kit, EIF2BA ELISA Kit, eIF-2a ELISA Kit, translation initiation factor eIF-2B alpha subunit ELISA Kit, hypothetical protein ELISA Kit, Translation initiation factor eIF-2B subunit alpha ELISA Kit, eukaryotic translation initiation factor 2B subunit alpha ELISA Kit, eukaryotic translation initiation factor 2B, subunit 1 (alpha) ELISA Kit, eIF2b1 ELISA Kit, PGTG_05021 ELISA Kit, ei2ba ELISA Kit, eif2b1 ELISA Kit, Eif2b1 ELISA Kit, EIF2B1 ELISA Kit
    基因ID
    1965
    UniProt
    P05198
    途径
    Methionine Biosynthetic Process
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