E-cadherin ELISA 试剂盒
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- 抗原 See all E-cadherin (CDH1) ELISA试剂盒
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Mouse E-Cadherin SpeedELISA Kit.
- 品牌
- SpeedELISA
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product CDH1 ELISA Kit
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- 样本量
- 100 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - 有效期
- 6 months
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- 抗原 See all E-cadherin (CDH1) ELISA试剂盒
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- 别名
- E-Cadherin (CDH1 产品)
- 别名
- Arc-1 ELISA Kit, CD324 ELISA Kit, CDHE ELISA Kit, ECAD ELISA Kit, LCAM ELISA Kit, UVO ELISA Kit, E-cadherin ELISA Kit, cdh1 ELISA Kit, si:dz180o5.2 ELISA Kit, uvo ELISA Kit, lcam ELISA Kit, E-Cad ELISA Kit, XBcad ELISA Kit, l-cam ELISA Kit, xcdh1 ELISA Kit, cdhc-A ELISA Kit, xb-cad ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, uvomorulin ELISA Kit, XB-cadherin ELISA Kit, arc-1 ELISA Kit, cd324 ELISA Kit, cdhe ELISA Kit, ecad ELISA Kit, AA960649 ELISA Kit, E-cad ELISA Kit, Ecad ELISA Kit, L-CAM ELISA Kit, Um ELISA Kit, cadherin 1 ELISA Kit, si:busm1-180o5.2 ELISA Kit, cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit, cadherin 1, type 1 S homeolog ELISA Kit, CDH1 ELISA Kit, si:busm1-180o5.2 ELISA Kit, cdh1 ELISA Kit, Cdh1 ELISA Kit, cdh1.S ELISA Kit
- 基因ID
- 12550
- UniProt
- P09803
- 途径
- WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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