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SPOCK3 ELISA 试剂盒
SPOCK3
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
SPOCK3ELISA Kit详情
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SPOCK3
(Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Mouse Testican 3 (SPOCK) ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
The antibody pair provided in this kit recognizes mouse Testican 3/SPOCK.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Top Product
Discover our top product SPOCK3 IQ-ELISA Kit
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
SPOCK3目标详情
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SPOCK3
(Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
别名
Testican 3 (SPOCK3 产品 )
别名
im:7139568 ELISA Kit, si:dkey-285b22.1 ELISA Kit, HSAJ1454 ELISA Kit, TES-3 ELISA Kit, TICN3 ELISA Kit, Testican-3 ELISA Kit, 2900045C01Rik ELISA Kit, AI428471 ELISA Kit, mKIAA4039 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3 ELISA Kit, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 ELISA Kit, spock3 ELISA Kit, SPOCK3 ELISA Kit, Spock3 ELISA Kit
基因ID
72902
UniProt
Q8BKV0
途径
Glycosaminoglycan Metabolic Process
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