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COL18A1 ELISA 试剂盒

COL18A1 适用: 小鼠 Colorimetric Sandwich ELISA 2.458-600 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4885503
发货至: 中国
  • 抗原 See all COL18A1 ELISA试剂盒
    COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
    适用
    • 8
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    2.458-600 ng/mL
    最低检测浓度
    2.458 ng/mL
    应用范围
    ELISA
    原理
    Mouse Endostatin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Mouse Endostatin (COL18A1)
    灵敏度
    2.5 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our best selling COL18A1 ELISA Kit
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    Discover our top product COL18A1 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Pan, Luo, Zhou, Han, Zeng: "Potent antitumor activity of the combination of HSV-TK and endostatin by adeno-associated virus vector for bladder cancer in vivo." in: Clinical laboratory, Vol. 59, Issue 9-10, pp. 1147-58, (2013) (PubMed).

    Pan, Zhou, Zeng, Han: "Suppression of bladder cancer growth in mice by adeno-associated virus vector-mediated endostatin expression." in: Tumour biology, Vol. 32, Issue 2, pp. 301-10, (2011) (PubMed).

  • 抗原 See all COL18A1 ELISA试剂盒
    COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
    别名
    Endostatin (COL18A1 产品)
    别名
    KNO ELISA Kit, KNO1 ELISA Kit, KS ELISA Kit, cb371 ELISA Kit, cb529 ELISA Kit, fc96d06 ELISA Kit, sb:cb529 ELISA Kit, wu:fb47h10 ELISA Kit, wu:fb77g12 ELISA Kit, wu:fc50f04 ELISA Kit, wu:fc96d06 ELISA Kit, wu:fk02h12 ELISA Kit, kno ELISA Kit, kno1 ELISA Kit, collagen type XVIII alpha 1 chain ELISA Kit, collagen type XVIII alpha 1 chain a ELISA Kit, collagen, type XVIII, alpha 1 ELISA Kit, collagen type XVIII alpha 1 ELISA Kit, COL18A1 ELISA Kit, col18a1a ELISA Kit, Col18a1 ELISA Kit, col18a1 ELISA Kit
    物质类
    Chemical
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