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BAD ELISA 试剂盒
BAD
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
BADELISA Kit详情
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抗原
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BAD
(BCL2-Associated Agonist of Cell Death (BAD))
适用
All reactivities for BAD ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Mouse BAD ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
The antibody pair provided in this kit recognizes Mouse BAD.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
BAD目标详情
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抗原
See all BAD ELISA试剂盒
BAD
(BCL2-Associated Agonist of Cell Death (BAD))
别名
Bad (BAD 产品 )
别名
bad ELISA Kit, fa01b12 ELISA Kit, wu:fa01b12 ELISA Kit, wu:fa96d04 ELISA Kit, BAD ELISA Kit, BBC2 ELISA Kit, BCL2L8 ELISA Kit, AI325008 ELISA Kit, Bbc2 ELISA Kit, BCL2-associated agonist of cell death b ELISA Kit, BCL2 associated agonist of cell death ELISA Kit, BCL2-associated agonist of cell death ELISA Kit, badb ELISA Kit, BAD ELISA Kit, Bad ELISA Kit
基因ID
12015
UniProt
Q61337
途径
MAPK Pathway , PI3K-Akt Signaling , RTK signaling , Apoptosis , Fc-epsilon Receptor Signaling Pathway , Positive Regulation of Peptide Hormone Secretion , Carbohydrate Homeostasis , Positive Regulation of Endopeptidase Activity , Regulation of Carbohydrate Metabolic Process , Hepatitis C , CXCR4-mediated Signaling Events
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