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FGF1 ELISA 试剂盒
FGF1
适用: 小鼠
Colorimetric
Sandwich ELISA
12-3000 pg/mL
Cell Culture Supernatant, Plasma, Serum
FGF1ELISA Kit详情
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FGF1
(Fibroblast Growth Factor 1 (Acidic) (FGF1))
适用
All reactivities for FGF1 ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
12-3000 pg/mL
最低检测浓度
12 pg/mL
应用范围
ELISA
原理
Mouse aFGF (FGF-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
The antibody pair provided in this kit recognizes Mouse Fibroblast Growth Factor acidic (FGF1)
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
FGF1目标详情
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抗原
See all FGF1 ELISA试剂盒
FGF1
(Fibroblast Growth Factor 1 (Acidic) (FGF1))
别名
aFGF (FGF1 产品 )
别名
AFGF ELISA Kit, ECGF ELISA Kit, ECGF-beta ELISA Kit, ECGFA ELISA Kit, ECGFB ELISA Kit, FGF-1 ELISA Kit, FGF-alpha ELISA Kit, FGFA ELISA Kit, GLIO703 ELISA Kit, HBGF-1 ELISA Kit, HBGF1 ELISA Kit, Fgf1 ELISA Kit, FGF1 ELISA Kit, aFGF ELISA Kit, ecgf ELISA Kit, fgfa ELISA Kit, ecgfa ELISA Kit, ecgfb ELISA Kit, fgf-1 ELISA Kit, hbgf1 ELISA Kit, glio703 ELISA Kit, ecgf-beta ELISA Kit, fgf-alpha ELISA Kit, Dffrx ELISA Kit, Fam ELISA Kit, Fgf-1 ELISA Kit, Fgfa ELISA Kit, zgc:136885 ELISA Kit, LOC100221393 ELISA Kit, fgf1 ELISA Kit, zgc:73249 ELISA Kit, fibroblast growth factor 1 ELISA Kit, fibroblast growth factor 1 L homeolog ELISA Kit, fibroblast growth factor 1b ELISA Kit, fibroblast growth factor 1a ELISA Kit, FGF1 ELISA Kit, fgf1.L ELISA Kit, fgf1 ELISA Kit, Fgf1 ELISA Kit, fgf1b ELISA Kit, LOC100221393 ELISA Kit, fgf1a ELISA Kit
基因ID
14164
UniProt
P61148
途径
RTK signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway
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