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CD130/gp130 ELISA 试剂盒

IL6ST 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4885339
发货至: 中国
  • 抗原 See all CD130/gp130 (IL6ST) ELISA试剂盒
    CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
    适用
    • 8
    • 7
    • 6
    • 3
    • 2
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human gp130 (sgp130) SpeedELISA Kit.
    品牌
    SpeedELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all CD130/gp130 (IL6ST) ELISA试剂盒
    CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
    别名
    Gp130 (IL6ST 产品)
    别名
    CD130 ELISA Kit, CDW130 ELISA Kit, GP130 ELISA Kit, IL-6RB ELISA Kit, 5133400A03Rik ELISA Kit, AA389424 ELISA Kit, BB405851 ELISA Kit, D13Ertd699e ELISA Kit, gp130 ELISA Kit, Ac1055 ELISA Kit, Gp130 ELISA Kit, Il-6rb ELISA Kit, IL-6 ELISA Kit, interleukin 6 signal transducer ELISA Kit, IL6ST ELISA Kit, Il6st ELISA Kit, il6st ELISA Kit
    基因ID
    3572
    UniProt
    P40189
    途径
    JAK/STAT Signaling, Cellular Glucan Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Cancer Immune Checkpoints
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