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TIMP2 ELISA 试剂盒

TIMP2 适用: 人 Colorimetric Sandwich ELISA 25-7000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4884806
发货至: 中国
  • 抗原 See all TIMP2 ELISA试剂盒
    TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    25-7000 pg/mL
    最低检测浓度
    25 pg/mL
    应用范围
    ELISA
    原理
    Human TIMP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, PDGF, SCF, TNF-alpha, TNF-beta, TPO, VEGF.
    灵敏度
    2 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling TIMP2 ELISA Kit
    Top Product
    Discover our top product TIMP2 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples300 - 3,000 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Prato, Khadjavi, Magnetto, Gulino, Rolfo, Todros, Cavalli, Guiot: "Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta." in: Bioscience, biotechnology, and biochemistry, Vol. 80, Issue 3, pp. 466-72, (2016) (PubMed).

    Gulino, Magnetto, Khadjavi, Panariti, Rivolta, Soster, Argenziano, Cavalli, Giribaldi, Guiot, Prato: "Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes." in: Mediators of inflammation, Vol. 2015, pp. 964838, (2015) (PubMed).

    Garratt, Sutanto, Ling, Looi, Iosifidis, Martinovich, Shaw, Kicic-Starcevich, Knight, Ranganathan, Stick, Kicic: "Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).

    Khadjavi, Magnetto, Panariti, Argenziano, Gulino, Rivolta, Cavalli, Giribaldi, Guiot, Prato: "Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing." in: Toxicology and applied pharmacology, Vol. 286, Issue 3, pp. 198-206, (2015) (PubMed).

    Ciaffi, Cavassini, Genne, Delhumeau, Spycher Elbes, Hill, Wandeler, Fehr, Stoeckle, Schmid, Hirschel, Montecucco, Calmy: "Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study." in: European journal of clinical investigation, Vol. 45, Issue 7, pp. 720-30, (2015) (PubMed).

    Basilico, Magnetto, DAlessandro, Panariti, Rivolta, Genova, Khadjavi, Gulino, Argenziano, Soster, Cavalli, Giribaldi, Guiot, Prato: "Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium." in: Toxicology and applied pharmacology, Vol. 288, Issue 3, pp. 330-8, (2015) (PubMed).

    Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

    Kiliç, Uçar, Özdemir, Colak, Bal, Ertu?rul: "Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions." in: Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, Vol. 14, Issue 6, pp. 531-41, (2014) (PubMed).

    Richards, Timulak, Doherty, Sharry, Colla, Joyce, Hayes: "Internet-delivered treatment: its potential as a low-intensity community intervention for adults with symptoms of depression: protocol for a randomized controlled trial." in: BMC psychiatry, Vol. 14, pp. 147, (2014) (PubMed).

    Hampel, Klonisch, Sel, Schulze, Garreis, Seitmann, Zouboulis, Paulsen: "Insulin-like factor 3 promotes wound healing at the ocular surface." in: Endocrinology, Vol. 154, Issue 6, pp. 2034-45, (2013) (PubMed).

    Chon, Kim, Ahn: "Transient elastography in real practice: a time for action?" in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1134-5, (2012) (PubMed).

    Kaminska-Winciorek, Deja, Polanska, Jarosz-Chobot: "The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study." in: International journal of clinical practice, Vol. 66, Issue 4, pp. 374-7, (2012) (PubMed).

    Müller, Kainov, El Bakkouri, Saelens, De Brabander, Kittel, Samm, Muller: "The proton translocation domain of cellular vacuolar ATPase provides a target for the treatment of influenza A virus infections." in: British journal of pharmacology, Vol. 164, Issue 2, pp. 344-57, (2012) (PubMed).

    Mamlouk, Balagurumoorthy, Wang, Adelstein, Kassis: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." in: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).

    Moir, Ng, Poniris, Santa, Burgess, Oliver, Krymskaya, Black: "Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells." in: British journal of pharmacology, Vol. 164, Issue 1, pp. 83-92, (2012) (PubMed).

    Steed, Trumpower, Duffy, Smith, Marshall, Rupp, Robson: "Amnion-derived cellular cytokine solution: a physiological combination of cytokines for wound healing." in: Eplasty, Vol. 8, pp. e18, (2008) (PubMed).

    Malard, Berenguer, Prat, Ruat, Steinmetz, Quemeneur: "Global gene expression profiling in human lung cells exposed to cobalt." in: BMC genomics, Vol. 8, pp. 147, (2007) (PubMed).

    Burns, Griffith, Waldhausen: "A linear oscillating electromotor for possible application in an intrathoracic artificial heart." in: Transactions - American Society for Artificial Internal Organs, Vol. 10, pp. 151-3 (PubMed).

    Hickman, Sargent: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." in: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).

    Jay: "Production of lysozyme by staphylococci and its correlation with three other extracellular substances." in: Journal of bacteriology, Vol. 91, Issue 5, pp. 1804-10 (PubMed).

  • 抗原 See all TIMP2 ELISA试剂盒
    TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
    别名
    TIMP-2 (TIMP2 产品)
    别名
    CSC-21K ELISA Kit, DDC8 ELISA Kit, timp2l ELISA Kit, zgc:65967 ELISA Kit, zgc:85799 ELISA Kit, D11Bwg1104e ELISA Kit, Timp-2 ELISA Kit, TIMP-2 ELISA Kit, etID32613.12 ELISA Kit, fa96h09 ELISA Kit, timp2 ELISA Kit, wu:fa96h09 ELISA Kit, zgc:136726 ELISA Kit, TIMP metallopeptidase inhibitor 2 ELISA Kit, TIMP metallopeptidase inhibitor 2b ELISA Kit, tissue inhibitor of metalloproteinase 2 ELISA Kit, TIMP metallopeptidase inhibitor 2a ELISA Kit, TIMP metallopeptidase inhibitor 2 L homeolog ELISA Kit, TIMP2 ELISA Kit, timp2b ELISA Kit, Timp2 ELISA Kit, timp2a ELISA Kit, timp2.L ELISA Kit
    背景
    Gene Names: TIMP2
    Protein names: Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)
    基因ID
    7077
    UniProt
    P16035
    途径
    cAMP Metabolic Process
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