CCL3 ELISA 试剂盒
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- 抗原 See all CCL3 ELISA试剂盒
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6-3000 pg/mL
- 最低检测浓度
- 6 pg/mL
- 应用范围
- ELISA
- 原理
- Human MIP-1 alpha (CCL3) ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 6 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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Collect plasma using heparin and/or EDTA as the anticoagulant. Citrate is not recommended.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays." in: Journal of neuroimmunology, Vol. 293, pp. 39-44, (2016) (PubMed).
: "The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery." in: International journal of cancer, Vol. 138, Issue 11, pp. 2688-99, (2016) (PubMed).
: "Genetic polymorphism of CCL2-2510 and susceptibility to enterovirus 71 encephalitis in a Chinese population." in: Archives of virology, Vol. 159, Issue 9, pp. 2503-7, (2014) (PubMed).
: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).
: "[Metal ceramic boundary surfaces in the Ni-Cr-ceramic system from a recent viewpoint]." in: Deutsche zahnärztliche Zeitschrift, Vol. 37, Issue 11, pp. 896-9, (1983) (PubMed).
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Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays." in: Journal of neuroimmunology, Vol. 293, pp. 39-44, (2016) (PubMed).
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- 抗原 See all CCL3 ELISA试剂盒
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- 别名
- MIP-1 alpha (CCL3 产品)
- 别名
- MIP-1alpha ELISA Kit, G0S19-1 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, MIP-1a ELISA Kit, Scya3 ELISA Kit, AI323804 ELISA Kit, LD78alpha ELISA Kit, MIP1-(a) ELISA Kit, MIP1-alpha ELISA Kit, Mip1a ELISA Kit, CCL3L1 ELISA Kit, CCL3L3 ELISA Kit, chemokine (C-C motif) ligand 3 ELISA Kit, C-C motif chemokine ligand 3 ELISA Kit, CCL3 ELISA Kit, Ccl3 ELISA Kit
- 背景
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Gene Names: CCL3 G0S19-1 MIP1A SCYA3
Protein names: C-C motif chemokine 3 (G0/G1 switch regulatory protein 19-1) (Macrophage inflammatory protein 1-alpha) (MIP-1-alpha) (PAT 464.1) (SIS-beta) (Small-inducible cytokine A3) (Tonsillar lymphocyte LD78 alpha protein) [Cleaved into: MIP-1-alpha(4-69) (LD78-alpha(4-69))]
- 基因ID
- 6348
- UniProt
- P10147
- 途径
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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