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LILRB4 ELISA 试剂盒
LILRB4
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
LILRB4ELISA Kit详情
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LILRB4
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))
适用
All reactivities for LILRB4 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Human LILRB4/CD85k/ILT-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human LILRB4.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
LILRB4目标详情
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LILRB4
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))
别名
LILRB4 (CD85k/ILT-3) (LILRB4 产品 )
别名
CD85K ELISA Kit, ILT-3 ELISA Kit, ILT3 ELISA Kit, LIR-5 ELISA Kit, LIR5 ELISA Kit, Gp49b ELISA Kit, HM18 ELISA Kit, gp49 ELISA Kit, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 ELISA Kit, leukocyte immunoglobulin like receptor B4 ELISA Kit, leukocyte immunoglobulin-like receptor, subfamily B, member 4A ELISA Kit, LILRB4 ELISA Kit, Lilrb4a ELISA Kit
基因ID
11006
UniProt
Q8NHJ6
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