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BMPR1B ELISA 试剂盒

BMPR1B 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4881803
发货至: 中国
  • 抗原 See all BMPR1B ELISA试剂盒
    BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human ALK-6/BMPR-IB ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human ALK-6.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all BMPR1B ELISA试剂盒
    BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))
    别名
    ALK-6 (BMPR1B 产品)
    别名
    ALK6 ELISA Kit, BMPR-IB ELISA Kit, FecB ELISA Kit, alk-6 ELISA Kit, alk6 ELISA Kit, cdw293 ELISA Kit, alk6b ELISA Kit, zgc:172219 ELISA Kit, BMPR1B ELISA Kit, AI385617 ELISA Kit, ALK-6 ELISA Kit, AV355320 ELISA Kit, Acvrlk6 ELISA Kit, Alk6 ELISA Kit, BMPR-1B ELISA Kit, CFK-43a ELISA Kit, SKR6 ELISA Kit, BR1b ELISA Kit, alk6tr ELISA Kit, bmpr1b ELISA Kit, zALK-6 ELISA Kit, zgc:92220 ELISA Kit, CDw293 ELISA Kit, BMP15 ELISA Kit, BMPRIB ELISA Kit, RPK-1 ELISA Kit, bone morphogenetic protein receptor type 1B ELISA Kit, bone morphogenetic protein receptor, type IBb ELISA Kit, bone morphogenetic protein receptor, type 1B ELISA Kit, bone morphogenetic protein receptor, type IBa ELISA Kit, BMPR1B ELISA Kit, bmpr1b ELISA Kit, bmpr1bb ELISA Kit, Bmpr1b ELISA Kit, bmpr1ba ELISA Kit
    基因ID
    658
    UniProt
    O00238
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