IGF1 ELISA 试剂盒 (Insulin-Like Growth Factor 1)

Details for Product IGF1 ELISA Kit No. ABIN4881446, 供应商: Log in to see
抗原
  • IGF-I
  • IGF1A
  • IGFI
  • C730016P09Rik
  • Igf-1
  • Igf-I
  • Npt2B
  • IGF-1
  • IGFIA
  • IGF-IB
  • igf1
  • IGF-1L
  • IGF-1a
  • IGF1
  • igf-1
  • igf1-A
  • igf1.S
  • xigf1
  • insulin like growth factor 1
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin like growth factor 1 L homeolog
  • IGF1
  • Igf1
  • LOC100136741
  • igf1
  • LOC678666
  • igf1.L
适用
牛, 人
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实验类型
Sandwich ELISA
检测范围
1.23-300 ng/mL
最低检测浓度
1.23 ng/mL
应用范围
ELISA
选项
Supplier
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原理 Bovine IGF-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
样品类型 Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
检测方法 Colorimetric
特异性 This ELISA antibody pair recognizes Bovine IGF-1.
产品特性
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
组件
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
试剂未包括
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others IGF1 products on genomics-online (e.g. as negative or positive controls)
抗原
别名 IGF-1 (IGF1 ELISA Kit 摘要)
基因ID 281239
UniProt P07455
途径 RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
应用备注 Recommended Dilution for serum and plasma samples2 fold
样本量 100 μL
板类型 Pre-coated
实验流程
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
限制 仅限研究用
储存条件 -20 °C
储存方法 The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期 6 months
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