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Luteinizing Hormone ELISA 试剂盒

LH 适用: 小鼠 Colorimetric Competition ELISA 370.4 pg/mL - 30000 pg/mL Plasma, Serum
产品编号 ABIN415551
发货至: 中国
  • 抗原 See all Luteinizing Hormone (LH) ELISA试剂盒
    Luteinizing Hormone (LH)
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    370.4 pg/mL - 30000 pg/mL
    最低检测浓度
    370.4 pg/mL
    应用范围
    ELISA
    原理
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of luteinizing hormone in mouse serum, plasma.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性

    This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).
    No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

    交叉反应 (详细)
    No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.
    灵敏度
    145.2 pg/mL
    组件
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Featured
    Discover our best selling LH ELISA Kit
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    Discover our top product LH ELISA Kit
  • 应用备注
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    说明

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    样本量
    50 μL
    实验时间
    2 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      Then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    试剂准备
    • Bring all kit components and samples to room temperature (18-25°C) before use.
    • Standard - Reconstitute the Standard with 0.5mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 30,000pg/mL. Please prepare 5 tubes containing 0.6mL Standard Diluent and produce a triple dilution series. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 30,000pg/mL, 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
    • Assay Diluent A and Assay Diluent B - Dilute 6mL of Assay Diluent A or B Concentrate(2×) with 6mL of deionized or distilled water to prepare 12 mL of Assay Diluent A or B. (In fact, more than 6mL Assay Diluent A and Assay Diluent B are contained in the bottles. Therefore, in every test, please precisely pipette required amount of Diluent and make double dilution in a new container. The prepared working dilution cannot be frozen.)
    • Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with working Assay Diluent A or B, respectively (1:100).
    • Wash Solution - Dilute 20mL of Wash Solution concentrate (30×) with 580mL of deionized or distilled water to prepare 600 mL of Wash Solution (1×).
    • TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.
    Note:
    • Making serial dilution in the wells directly is not permitted.
    • Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37°C directly.
    • Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
    • Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL for once pipetting.
    • The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    • If crystals have formed in the Wash Solution concentrate (30×), warm to room temperature and mix gently until the crystals are completely dissolved.
    • Contaminated water or container for reagent preparation will influence the detection result.
    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制
    仅限研究用
  • 注意事项
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    注意事项
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    储存条件
    4 °C
    储存方法
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    有效期
    6 months
  • Jalalie, Rezaee, Rezaie, Jalili, Raoofi, Rustamzade: "Human umbilical cord mesenchymal stem cells improve morphometric and histopathologic changes of cyclophosphamide-injured ovarian follicles in mouse model of premature ovarian failure." in: Acta histochemica, Vol. 123, Issue 1, pp. 151658, (2020) (PubMed).

    Shishikura, Kuroha, Matsueda, Iseki, Matsui, Inoue, Arita: "Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic processes in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, Issue 12, pp. 14194-14203, (2020) (PubMed).

    Cao, Liu, Zhang, Wang, Xiong, Wu, Chen: "Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling." in: Chemosphere, Vol. 244, pp. 125497, (2020) (PubMed).

    Tang, Zhang, Liu, Zhou, Hu, Zhong, Wang, Chen: "Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice." in: Neurotoxicology, Vol. 79, pp. 164-176, (2020) (PubMed).

    Li, Ma, Liu: "Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LβT2 Mouse Pituitary Cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 162, Issue 1, pp. 43-52, (2019) (PubMed).

    Fu, Chen, Xu, Ji, Zhang, Wang, Yu, Xu: "Vitamin D deficiency impairs testicular development and spermatogenesis in mice." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 73, pp. 241-249, (2018) (PubMed).

    Ding, Tan, Song, Ma, Xiao, Zhang: "Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring." in: Reproduction (Cambridge, England), Vol. 154, Issue 4, pp. 433-444, (2018) (PubMed).

    Ye, Li, Zhang, Ma, Ji, Huang, Curry, Liu, Liu: "Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis." in: Environmental science & technology, Vol. 51, Issue 17, pp. 10212-10221, (2018) (PubMed).

    Wang, Wang, Lu, Cui, Li, Li, Zhang, Zhang, Liu: "The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy-induced osteoporotic mice." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 36, Issue 5, pp. 1415-1424, (2018) (PubMed).

    Cao, Hua, Xiong, Zhu, Zhang, Chen: "Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice." in: Frontiers in molecular neuroscience, Vol. 11, pp. 6, (2018) (PubMed).

    Tang, Yin, Zhang, Chen, Jin, Liu: "Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population." in: Environmental pollution (Barking, Essex : 1987), Vol. 237, pp. 581-591, (2018) (PubMed).

    Chin, Wang, Hsieh, Shih, Nana, Changou, Yang, Chiu, Fu, Davis, Tang, Lin: "Leptin OB3 peptide suppresses leptin-induced signaling and progression in ovarian cancer cells." in: Journal of biomedical science, Vol. 24, Issue 1, pp. 51, (2018) (PubMed).

    Yang, Chin, Hsieh, Lai, Ke, Crawford, Lee, Fu, Mousa, Grasso, Liu, Chang, Tang, Lin, Davis: "Novel leptin OB3 peptide-induced signaling and progression in thyroid cancers: Comparison with leptin." in: Oncotarget, Vol. 7, Issue 19, pp. 27641-54, (2018) (PubMed).

    Varamini, Mansfeld, Giddam, Steyn, Toth: "New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency." in: International journal of pharmaceutics, Vol. 521, Issue 1-2, pp. 327-336, (2017) (PubMed).

    Miura, Ohtani, Hasegawa, Yoshioka, Hwang: "High sensitivity of testicular function to titanium nanoparticles." in: The Journal of toxicological sciences, Vol. 42, Issue 3, pp. 359-366, (2017) (PubMed).

    Iizuka-Hishikawa, Hishikawa, Sasaki, Takubo, Goto, Nagata, Nakanishi, Shindou, Okamura, Ito, Toshimori, Sasaki, Shimizu: "Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis." in: The Journal of biological chemistry, Vol. 292, Issue 29, pp. 12065-12076, (2017) (PubMed).

    Varamini, Rafiee, Giddam, Mansfeld, Steyn, Toth: "Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity." in: Journal of medicinal chemistry, Vol. 60, Issue 20, pp. 8309-8320, (2017) (PubMed).

    Chen, Chen, Tsai, Tzai, Chen, Tsai: "Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy." in: Anticancer research, Vol. 37, Issue 12, pp. 6791-6797, (2017) (PubMed).

    Liu, Su, Hao, Chen, Liu, Liao, Li: "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." in: International journal of molecular sciences, Vol. 17, Issue 1, (2016) (PubMed).

    Spilker, Nullmeier, Grochowska, Schumacher, Butnaru, Macharadze, Gomes, Yuanxiang, Bayraktar, Rodenstein, Geiseler, Kolodziej, Lopez-Rojas, Montag, Angenstein, Bär, DHanis, Roskoden, Mikhaylova et al.: "A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis. ..." in: PLoS genetics, Vol. 12, Issue 3, pp. e1005907, (2016) (PubMed).

  • 抗原 See all Luteinizing Hormone (LH) ELISA试剂盒
    Luteinizing Hormone (LH)
    Abstract
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