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TNFRSF1B ELISA 试剂盒

TNFRSF1B 适用: 人 AA 23-257 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
产品编号 ABIN411364
发货至: 中国
  • 抗原 See all TNFRSF1B ELISA试剂盒
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    抗原表位
    AA 23-257
    适用
    • 5
    • 5
    • 3
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    7.8-500 pg/mL
    最低检测浓度
    7.8 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human sTNFsR???
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: L23-D257
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <2pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: L23-D257
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 4 TNFR-Cys repeats.

    板类型
    Pre-coated
    实验流程
    human sTNFsRII ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sTNFsRII has been precoated onto 96-well plates. Standards(NSO, L23-D257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sTNFsRII is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sTNFsRII amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human sTNFsRII standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human sTNFsRII standard solution and each sample is measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 63, Standard deviation: 3.09, CV(%): 4.9
    • Sample 2: n=16, Mean(pg/ml): 125, Standard deviation: 7.13, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 257, Standard deviation: 9.25, CV(%): 3.6,
    • Sample 1: n=24, Mean(pg/ml): 78, Standard deviation: 4.76, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 134, Standard deviation: 9.65, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 269, Standard deviation: 14, CV(%): 5.2
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all TNFRSF1B ELISA试剂盒
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    别名
    TNFRSF1B (TNFRSF1B 产品)
    别名
    CD120b ELISA Kit, TBPII ELISA Kit, TNF-R-II ELISA Kit, TNF-R75 ELISA Kit, TNFBR ELISA Kit, TNFR1B ELISA Kit, TNFR2 ELISA Kit, TNFR80 ELISA Kit, p75 ELISA Kit, p75TNFR ELISA Kit, TNF-RII/TNFRSF1B ELISA Kit, TNFR-II ELISA Kit, TNFRSF1B ELISA Kit, TNF-R2 ELISA Kit, TNF-alphaR2 ELISA Kit, TNFRII ELISA Kit, TNFalpha-R2 ELISA Kit, Tnfr-1 ELISA Kit, Tnfr2 ELISA Kit, rank ELISA Kit, zgc:163064 ELISA Kit, TNF receptor superfamily member 1B ELISA Kit, tumor necrosis factor receptor superfamily, member 1b ELISA Kit, tumor necrosis factor receptor superfamily, member 1B ELISA Kit, TNFRSF1B ELISA Kit, Tnfrsf1b ELISA Kit, tnfrsf1b ELISA Kit
    背景

    Protein Function: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. .

    Background: Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75- kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.

    Synonyms: Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2,TNF-R2,Tumor necrosis factor receptor type II,TNF-RII,TNFR-II,p75,p80 TNF-alpha receptor,CD120b,Etanercept,Tumor necrosis factor receptor superfamily member 1b, membrane form,Tumor necrosis factor-binding protein 2,TBP-2,TBPII,TNFRSF1B,TNFBR, TNFR2,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 1B

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    基因ID
    7133
    UniProt
    P20333
    途径
    NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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