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Caspase 3 ELISA 试剂盒

CASP3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
产品编号 ABIN3163894
发货至: 中国
  • 抗原 See all Caspase 3 (CASP3) ELISA试剂盒
    Caspase 3 (CASP3)
    适用
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.15 ng/mL - 10 ng/mL
    最低检测浓度
    0.15 ng/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP3 in human tissue homogenates, cell lysates, cell culture supernates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性

    This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3).
    No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.

    交叉反应 (详细)
    No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.
    灵敏度
    0.056 ng/mL
    组件
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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  • 应用备注
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    说明

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    样本量
    100 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    试剂准备
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 20 ng/mL. Firstly dilute the stock solution to 10 ng/mL and the diluted standard serves as the highest standard (10 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制
    仅限研究用
  • 注意事项
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    注意事项
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    储存条件
    4 °C
    储存方法
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    有效期
    6 months
  • Kurakula, Naveen: "In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma." in: Marine drugs, Vol. 18, Issue 4, (2021) (PubMed).

    Hacioglu, Kar, Kacar, Sahinturk, Kanbak: "High Concentrations of Boric Acid Trigger Concentration-Dependent Oxidative Stress, Apoptotic Pathways and Morphological Alterations in DU-145 Human Prostate Cancer Cell Line." in: Biological trace element research, Vol. 193, Issue 2, pp. 400-409, (2020) (PubMed).

    Alhakamy, Ahmed, Aldawsari, Alfaifi, Eid, Abdel-Naim, Fahmy: "Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells." in: International journal of molecular sciences, Vol. 20, Issue 22, (2020) (PubMed).

    Fahmy, Fahmy: "In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells." in: Photodiagnosis and photodynamic therapy, Vol. 30, pp. 101714, (2020) (PubMed).

    Hosny, Rizg, Khallaf: "Preparation and Optimization of In Situ Gel Loaded with Rosuvastatin-Ellagic Acid Nanotransfersomes to Enhance the Anti-Proliferative Activity." in: Pharmaceutics, Vol. 12, Issue 3, (2020) (PubMed).

    Zuo, Li, Jiang, Lan, Tang, Kang, Zou, Wang, Zhang, Tang: "Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus." in: Frontiers in neuroscience, Vol. 14, pp. 169, (2020) (PubMed).

    Alhakamy, Fahmy, Ahmed, Caruso, Caraci, Asfour, Bakhrebah, N Alomary, Abdulaal, Okbazghi, Abdel-Naim, Eid, Aldawsari, Kurakula, Mohamed: "Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity." in: Marine drugs, Vol. 18, Issue 4, (2020) (PubMed).

    Al-Wahaibi, Al-Saleem, Ahmed, Fahmy, Alhakamy, Eid, Abdel-Naim, Abdel-Mageed, AlRasheed, Shazly: "Optimized Conjugation of Fluvastatin to HIV-1 TAT Displays Enhanced Pro-Apoptotic Activity in HepG2 Cells." in: International journal of molecular sciences, Vol. 21, Issue 11, (2020) (PubMed).

    Alhakamy, Ahmed, Kurakula, Caruso, Caraci, Asfour, Alfarsi, Eid, Mohamed, Alruwaili, Abdulaal, Fahmy, Alhadrami, Eldakhakhny, Abdel-Naim: "Chitosan-Based Microparticles Enhance Ellagic Acid's Colon Targeting and Proapoptotic Activity." in: Pharmaceutics, Vol. 12, Issue 7, (2020) (PubMed).

    Degroote, Vergauwen, Van Noten, Wang, De Smet, Van Ginneken, Michiels: "The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets." in: Antioxidants (Basel, Switzerland), Vol. 8, Issue 8, (2019) (PubMed).

    Forte, Torricelli, Bonvicini, Boanini, Gentilomi, Lusvardi, Della Bella, Fini, Vecchio Nepita, Bigi: "Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine." in: Journal of inorganic biochemistry, Vol. 178, pp. 43-53, (2018) (PubMed).

    Attia, Nounou, Shalaby: "Zinc Oxide Nanoparticles Induced Oxidative DNA Damage, Inflammation and Apoptosis in Rat's Brain after Oral Exposure." in: Toxics, Vol. 6, Issue 2, (2018) (PubMed).

    Chen, Chen, Huang, Huang, Wang, Hsieh, Huang, Liu, Shiu: "Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells." in: The American journal of Chinese medicine, Vol. 45, Issue 2, pp. 351-368, (2017) (PubMed).

    Chen, Huang, Huang, Liu, Lin, Liu, Hsieh, Shiu, Chen: "Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells." in: BMC cancer, Vol. 16, pp. 532, (2016) (PubMed).

    Elshal, Abu-Elsaad, El-Karef, Ibrahim: "The multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosis." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 388, Issue 12, pp. 1293-304, (2015) (PubMed).

    Pradeep, Suke, Prasad, Singh, Martande, Nagpal, Naik, Guruprasad, Raju, Singh, Siddaya: "Expression of key executioner of apoptosis caspase-3 in periodontal health and disease." in: Journal of investigative and clinical dentistry, (2014) (PubMed).

    El Morsy, Ahmed, Ahmed: "Attenuation of renal ischemia/reperfusion injury by açaí extract preconditioning in a rat model." in: Life sciences, (2014) (PubMed).

  • 抗原 See all Caspase 3 (CASP3) ELISA试剂盒
    Caspase 3 (CASP3)
    别名
    CASP3 (CASP3 产品)
    别名
    CPP32 ELISA Kit, CPP32B ELISA Kit, SCA-1 ELISA Kit, A830040C14Rik ELISA Kit, AC-3 ELISA Kit, Apopain ELISA Kit, CC3 ELISA Kit, Caspase-3 ELISA Kit, Lice ELISA Kit, Yama ELISA Kit, mldy ELISA Kit, xcpp32 ELISA Kit, casp3 ELISA Kit, zgc:100890 ELISA Kit, CASP-3 ELISA Kit, caspase-3 ELISA Kit, caspase 3 ELISA Kit, caspase 3 S homeolog ELISA Kit, caspase 3, apoptosis-related cysteine peptidase a ELISA Kit, caspase 3, apoptosis-related cysteine peptidase ELISA Kit, CASP3 ELISA Kit, Casp3 ELISA Kit, casp3.S ELISA Kit, casp3a ELISA Kit
    UniProt
    P42574
    途径
    Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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