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Vip ELISA 试剂盒

Vip 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN3159060
发货至: 中国
  • 抗原 See all Vip ELISA试剂盒
    Vip (Vasoactive Intestinal Peptide (Vip))
    适用
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
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    • 1
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    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    6.17 pg/mL - 500 pg/mL
    最低检测浓度
    6.17 pg/mL
    应用范围
    ELISA
    原理
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of VIP in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性

    This assay has high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP).
    No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.

    交叉反应 (详细)
    No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.
    灵敏度
    2.63 pg/mL
    组件
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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  • 应用备注
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    说明

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    样本量
    50 μL
    实验时间
    2 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      Then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    试剂准备
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 500pg/mL. Please prepare 5 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 500pg/mL, 166.67pg/mL, 55.56pg/mL, 18.52pg/mL, 6.17pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
    4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制
    仅限研究用
  • 注意事项
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    注意事项
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    储存条件
    4 °C
    储存方法
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    有效期
    6 months
  • Haririan, Andrukhov, Böttcher, Pablik, Wimmer, Moritz, Rausch-Fan: "Salivary neuropeptides, stress, and periodontitis." in: Journal of periodontology, Vol. 89, Issue 1, pp. 9-18, (2018) (PubMed).

    Ge, Duan, Yang, Zhang, Zhang, Wang, Yang, Sun, Zhang, Su, Zhu, Zhou, Liu, Shi, Yu, Yang, Chang, Zhang, Wu, Chen: "Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2086163, (2018) (PubMed).

    Peng, Liu, Hu, Yan, Gao, Wu: "Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut." in: Scientific reports, Vol. 7, pp. 40278, (2017) (PubMed).

    McDonald, Smith, Smith, Changli Xue, Golianu, Cripps: "Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 116, Issue 6, pp. 497-505, (2016) (PubMed).

    Riesco, Cernuda-Morollón, Martínez-Camblor, Pérez-Alvarez, Verano, García-Cabo, Serrano-Pertierra, Pascual: "Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients." in: Cephalalgia : an international journal of headache, (2016) (PubMed).

    Cernuda-Morollón, Riesco, Martínez-Camblor, Serrano-Pertierra, García-Cabo, Pascual: "No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine." in: Headache, Vol. 56, Issue 9, pp. 1448-1454, (2016) (PubMed).

    Cernuda-Morollón, Martínez-Camblor, Alvarez, Larrosa, Ramón, Pascual: "Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine." in: Cephalalgia : an international journal of headache, Vol. 35, Issue 4, pp. 310-6, (2015) (PubMed).

    Ul Kabir, Samad, Ahmed, Jahan, Akhter, Tasnim, Hasan, Sayfe, Hannan et al.: "Aqueous fraction of beta vulgaris ameliorates hyperglycemia in diabetic mice due to enhanced glucose stimulated insulin secretion, mediated by acetylcholine and GLP-1, and elevated glucose uptake via ..." in: PLoS ONE, Vol. 10, Issue 2, pp. e0116546, (2015) (PubMed).

    Xiong, Wang, Li, Tian, Zheng, Huang: "Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 5, pp. 775-81, (2014) (PubMed).

    Cernuda-Morollón, Martínez-Camblor, Ramón, Larrosa, Serrano-Pertierra, Pascual: "CGRP and VIP levels as predictors of efficacy of Onabotulinumtoxin type A in chronic migraine." in: Headache, Vol. 54, Issue 6, pp. 987-95, (2014) (PubMed).

    Corrêa, da Costa Rocha, de Sousa, do Carmo Pereira Nunes, Gollob, Dutra, da Silva Menezes: "Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy." in: Human immunology, Vol. 74, Issue 10, pp. 1375-81, (2013) (PubMed).

    Zhang, Zhang, Liu, Xiong, Ni, Hui, He, Pu: "VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population." in: Clinical genetics, Vol. 75, Issue 6, pp. 544-9, (2009) (PubMed).

  • 抗原 See all Vip ELISA试剂盒
    Vip (Vasoactive Intestinal Peptide (Vip))
    别名
    VIP (Vip 产品)
    别名
    PHM27 ELISA Kit, vip1 ELISA Kit, zgc:171463 ELISA Kit, VIP ELISA Kit, vip-A ELISA Kit, PHI ELISA Kit, vasoactive intestinal peptide ELISA Kit, vasoactive intestinal peptide L homeolog ELISA Kit, vasoactive intestinal polypeptide ELISA Kit, VIP ELISA Kit, Vip ELISA Kit, vip ELISA Kit, vip.L ELISA Kit
    UniProt
    P01282
    途径
    Hormone Activity, cAMP Metabolic Process
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