HMOX1 ELISA 试剂盒
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- 抗原 See all HMOX1 ELISA试剂盒
- HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))
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适用
- 人, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.781 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.781 ng/mL
- 应用范围
- ELISA
- 原理
- Colorimetric detection of heme oxygenase 1
- 样品类型
- Cell Lysate, Cyst Fluid, Serum, Tissue Samples
- Analytical Method
- Quantitative
- 灵敏度
- 0.21 ng/mL
- 产品特性
- ELISA kit used to quantitate HO-1 concentration in samples.
- 组件
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- Anti-HO-1 Immunoassay Plate
- 5X HO-1 Extraction Reagent
- Recombinant HO-1 Standard
- Standard and Sample Diluent
- 10X Wash Buffer Concentrate
- Anti-HO-1 Biotinylated Antibody Concentrate
- Anti-HO-1 Biotinylated Antibody Diluent
- Streptavidin: HRP Concentrate
- Streptavidin: HRP Diluent
- TMB Substrate
- Stop Solution
- Pre-treatment Buffer
- 试剂未包括
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- Ultra pure water
- Additional reagents and materials for cell lysate and tissue extract preparation, including protease inhibitors
- Precision pipettors, with disposable plastic tips
- Polypropylene or polyethylene tubes to prepare samples − do not use polystyrene, polycarbonate or glass tubes
- A container to prepare 1X Wash Buffer
- A wash bottle or an automated 96-well plate washer - Top Product
- Discover our top product HMOX1 ELISA Kit
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Heme Oxygenase (Decycling) 1 (HMOX1) ELISA Kit
HMOX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 AA 1-288 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 实验时间
- 0.5 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare Standard and samples in Standard and Sample Diluent.
- Add 50 μL of Pre-Treatment Buffer to all sample and standard wells.
- Add 50 μL of Standard and sample to appropriate wells.
- Cover plate with Plate Sealer and incubate at room temperature (20-25 °C) for 2 hours.
- Wash plate four times with 1X Wash Buffer.
- Add 100 μL of Biotinylated Antibody Working Solution to each well.
- Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
- Wash plate four times with 1X Wash Buffer as described in step
- 9. Add 100 μL of Streptavidin-HRP Working Solution to each well.
- Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
- Wash plate four times with 1X Wash Buffer as described in step
- 12. Add 100 μL of TMB Substrate to each well.
- Develop the plate in the dark at room temperature for 30 minutes.
- Stop reaction by adding 100 μL of Stop Solution to each well.
- Measure absorbance on a plate reader at 450 nm.
- 实验流程
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- Prepare Standard and samples in Standard and Sample Diluent.
- Add 50 μL of Pre-Treatment Buffer to all sample and standard wells.
- Add 50 μL of Standard and sample to appropriate wells.
- Cover plate with Plate Sealer and incubate at room temperature (20-25 °C) for 2 hours.
- Wash plate four times with 1X Wash Buffer.
- Add 100 μL of Biotinylated Antibody Working Solution to each well.
- Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
- Wash plate four times with 1X Wash Buffer as described in step
- 9. Add 100 μL of Streptavidin-HRP Working Solution to each well.
- Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
- Wash plate four times with 1X Wash Buffer as described in step
- 12. Add 100 μL of TMB Substrate to each well.
- Develop the plate in the dark at room temperature for 30 minutes.
- Stop reaction by adding 100 μL of Stop Solution to each well.
- Measure absorbance on a plate reader at 450 nm.
- 结果分析
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Duplicate absorbance values should be within 10% of each other. Care should be taken when interpreting data with differences in absorbance values greater than 10%.
1. Prepare a standard curve to determine the amount of HO-1 in an unknown sample. Plot the average absorbance obtained for each standard concentration on the vertical (Y) axis versus the corresponding HO-1 concentration on the horizontal (X) axis using graph paper or curve-fitting software.
2. Calculate the HO-1 concentration in unknown samples using the prepared standard curve. Determine the amount of HO-1 in each unknown sample by noting the HO-1 concentration (X axis) that correlates with the absorbance value (Y axis) obtained for the unknown sample.
3. Multiply the HO-1 concentration obtained by the dilution factor to determine the amount of HO-1 in the undiluted sample. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all HMOX1 ELISA试剂盒
- HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))
- 别名
- HO-1 (HMOX1 产品)
- 别名
- HMOX1 ELISA Kit, ARABIDOPSIS THALIANA HEME OXYGENASE 1 ELISA Kit, ATHO1 ELISA Kit, F18A8.4 ELISA Kit, F18A8_4 ELISA Kit, GENOMES UNCOUPLED 2 ELISA Kit, GUN2 ELISA Kit, HEME OXYGENASE ELISA Kit, HEME OXYGENASE 1 ELISA Kit, HEME OXYGENASE 6 ELISA Kit, HO1 ELISA Kit, HY1 ELISA Kit, HY6 ELISA Kit, PLASTID HEME OXYGENASE ELISA Kit, REVERSAL OF THE DET PHENOTYPE 4 ELISA Kit, HO-1 ELISA Kit, wu:fc27c04 ELISA Kit, zgc:65984 ELISA Kit, D8Wsu38e ELISA Kit, Hemox ELISA Kit, Hmox ELISA Kit, Hsp32 ELISA Kit, HEOXG ELISA Kit, Heox ELISA Kit, Ho-1 ELISA Kit, Ho1 ELISA Kit, hsp32 ELISA Kit, MGC132176 ELISA Kit, Hmox1 ELISA Kit, HMOX1D ELISA Kit, HSP32 ELISA Kit, bK286B10 ELISA Kit, heme oxygenase 1 ELISA Kit, Plant heme oxygenase (decyclizing) family protein ELISA Kit, Heme oxygenase ELISA Kit, heme oxygenase ELISA Kit, heme oxygenase 1, chloroplastic ELISA Kit, heme oxygenase 1a ELISA Kit, heme oxygenase 1 L homeolog ELISA Kit, HMOX1 ELISA Kit, TED4 ELISA Kit, ho1 ELISA Kit, P9301_RS17555 ELISA Kit, A9601_RS17590 ELISA Kit, MAE_RS06565 ELISA Kit, HO1 ELISA Kit, hmox1a ELISA Kit, CPE0214 ELISA Kit, Cyan7425_2962 ELISA Kit, Hmox1 ELISA Kit, hmox1.L ELISA Kit, AM1_C0205 ELISA Kit, CC1G_11686 ELISA Kit, CpipJ_CPIJ007246 ELISA Kit
- 背景
- Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin. These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs. There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals. HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation. It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress.
- 途径
- Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
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