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CD86 ELISA 试剂盒

CD86 适用: 小鼠 AA 26-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
产品编号 ABIN2859285
发货至: 中国
  • 抗原 See all CD86 ELISA试剂盒
    CD86
    抗原表位
    AA 26-245
    适用
    • 6
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5-4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD86/B7-2
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    NSO, V26-G245
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: V26-G245
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Expressed on activated B-cells.

    板类型
    Pre-coated
    实验流程
    mouse CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD86 has been precoated onto 96-well plates. Standards (NSO, V26-G245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD86 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD86 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD86 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(ng/ml): 0.21, Standard deviation: 0.011, CV(%): 5.3
    • Sample 2: n=16, Mean(ng/ml): 1.44, Standard deviation: 0.084, CV(%): 5.9
    • Sample 3: n=16, Mean(ng/ml): 2.59, Standard deviation: 0.161, CV(%): 6.2,
    • Sample 1: n=24, Mean(ng/ml): 0.23, Standard deviation: 0.013, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.095, CV(%): 6.5
    • Sample 3: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.150, CV(%): 6.1
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CD86 ELISA试剂盒
    CD86
    别名
    CD86 (CD86 产品)
    别名
    B7-2 ELISA Kit, B7.2 ELISA Kit, B70 ELISA Kit, CD28LG2 ELISA Kit, LAB72 ELISA Kit, B7 ELISA Kit, CLS1 ELISA Kit, Cd28l2 ELISA Kit, ETC-1 ELISA Kit, Ly-58 ELISA Kit, Ly58 ELISA Kit, MB7 ELISA Kit, MB7-2 ELISA Kit, TS/A-2 ELISA Kit, CD86 molecule ELISA Kit, CD86 antigen ELISA Kit, integrin subunit alpha 2b ELISA Kit, CD86 ELISA Kit, Cd86 ELISA Kit, Itga2b ELISA Kit
    背景

    Protein Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

    Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

    Synonyms: T-lymphocyte activation antigen CD86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1,CD86,Cd86,

    Full Gene Name: T-lymphocyte activation antigen CD86

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein.
    基因ID
    12524
    UniProt
    P42082
    途径
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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