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MMP 9 ELISA 试剂盒

MMP9 适用: 兔 Colorimetric Sandwich ELISA 61-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2748383
发货至: 中国
  • 抗原 See all MMP 9 (MMP9) ELISA试剂盒
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    61-15000 pg/mL
    最低检测浓度
    61 pg/mL
    应用范围
    ELISA
    原理
    Rabbit MMP-9 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Rabbit MMP-9
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples10 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all MMP 9 (MMP9) ELISA试剂盒
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    别名
    MMP-9 (MMP9 产品)
    别名
    CLG4B ELISA Kit, GELB ELISA Kit, MANDP2 ELISA Kit, MMP-9 ELISA Kit, AW743869 ELISA Kit, B/MMP9 ELISA Kit, Clg4b ELISA Kit, pro-MMP-9 ELISA Kit, mmp-9 ELISA Kit, fgMMP-9 ELISA Kit, mmp9 ELISA Kit, ZFMMP-9 ELISA Kit, wu:fb02g06 ELISA Kit, wu:fb07b05 ELISA Kit, wu:fi98c09 ELISA Kit, wu:fj05a08 ELISA Kit, zgc:64165 ELISA Kit, clg4b ELISA Kit, gelb ELISA Kit, mandp2 ELISA Kit, matrix metallopeptidase 9 ELISA Kit, collagenase ELISA Kit, matrix metalloproteinase 9 ELISA Kit, matrix metallopeptidase 9 S homeolog ELISA Kit, MMP9 ELISA Kit, RB3913 ELISA Kit, BPSS0666 ELISA Kit, Sbal_3732 ELISA Kit, Bcer98_0486 ELISA Kit, Shew185_0630 ELISA Kit, Shal_3056 ELISA Kit, Sbal195_0657 ELISA Kit, Lbys_3550 ELISA Kit, Palpr_2084 ELISA Kit, Mmp9 ELISA Kit, mmp9 ELISA Kit, mmp9.S ELISA Kit
    背景
    MMP-9
    基因ID
    100008993
    UniProt
    P41246
    途径
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
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