IL1RN ELISA 试剂盒
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- 抗原 See all IL1RN ELISA试剂盒
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rat Interleukin 1 Receptor antagonist (IL1RN / IL-1 F3) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Rat Interleukin 1 Receptor antagonist (IL1RN / IL-1 F3)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling IL1RN ELISA Kit
- Top Product
- Discover our top product IL1RN ELISA Kit
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Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kit
IL1RN 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 马 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 猴 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all IL1RN ELISA试剂盒
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
- 别名
- IL-1 Ra (IL1RN 产品)
- 别名
- DIRA ELISA Kit, ICIL-1RA ELISA Kit, IL-1RN ELISA Kit, IL-1ra ELISA Kit, IL-1ra3 ELISA Kit, IL1F3 ELISA Kit, IL1RA ELISA Kit, IRAP ELISA Kit, MVCD4 ELISA Kit, IRAP1 ELISA Kit, IL1RN ELISA Kit, IL-1RA ELISA Kit, F630041P17Rik ELISA Kit, interleukin 1 receptor antagonist ELISA Kit, interleukin 1 receptor antagonist-like ELISA Kit, IL1RN ELISA Kit, Il1rn ELISA Kit, IL1RNL ELISA Kit
- 背景
- Interleukin 1 Receptor antagonist (IL1RN / IL-1 F3)
- 基因ID
- 60582
- UniProt
- P25086
- 途径
- NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
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