CD130/gp130 ELISA 试剂盒
-
- 抗原 See all CD130/gp130 (IL6ST) ELISA试剂盒
- CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
-
适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rat gp130 (Il6st) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Rat gp130 (Il6st)
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling IL6ST ELISA Kit
- Top Product
- Discover our top product IL6ST ELISA Kit
-
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA Kit
IL6ST 适用: 人 Colorimetric Sandwich ELISA 0.938-60 ng/mL Plasma, Saliva, Serum, Tissue Homogenate, Urine
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 人 AA 24-619 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST 适用: 小鼠 AA 23-617 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
-
Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all CD130/gp130 (IL6ST) ELISA试剂盒
- CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
- 别名
- Gp130 (IL6ST 产品)
- 别名
- CD130 ELISA Kit, CDW130 ELISA Kit, GP130 ELISA Kit, IL-6RB ELISA Kit, 5133400A03Rik ELISA Kit, AA389424 ELISA Kit, BB405851 ELISA Kit, D13Ertd699e ELISA Kit, gp130 ELISA Kit, Ac1055 ELISA Kit, Gp130 ELISA Kit, Il-6rb ELISA Kit, IL-6 ELISA Kit, interleukin 6 signal transducer ELISA Kit, IL6ST ELISA Kit, Il6st ELISA Kit, il6st ELISA Kit
- 背景
- Gp130 (Il6st)
- UniProt
- P40190
- 途径
- JAK/STAT Signaling, Cellular Glucan Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Cancer Immune Checkpoints
-
