CD137 ELISA 试剂盒
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- 抗原 See all CD137 (TNFRSF9) ELISA试剂盒
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Rat 4-1BB (TNFRSF9/CD137) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Rat 4-1BB (TNFRSF9/CD137)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling TNFRSF9 ELISA Kit
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Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kit
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Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA KitTNFRSF9 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA KitTNFRSF9 适用: 小鼠 AA 24-187 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA KitTNFRSF9 适用: 人 AA 24-186 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA KitTNFRSF9 适用: 人 Colorimetric Sandwich ELISA 16-2000 pg/mL Cell Lysate, Plasma, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA KitTNFRSF9 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CD137 (TNFRSF9) ELISA试剂盒
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- 别名
- 4-1BB (TNFRSF9 产品)
- 别名
- TNFRSF9 ELISA Kit, zgc:136557 ELISA Kit, 4-1BB ELISA Kit, CD137 ELISA Kit, CDw137 ELISA Kit, ILA ELISA Kit, A930040I11Rik ELISA Kit, AA408498 ELISA Kit, AI325004 ELISA Kit, Cd137 ELISA Kit, Ly63 ELISA Kit, tumor necrosis factor receptor superfamily member 9 ELISA Kit, TNF receptor superfamily member 9 ELISA Kit, tumor necrosis factor receptor superfamily, member 9a ELISA Kit, tumor necrosis factor receptor superfamily, member 9 ELISA Kit, TNFRSF9 ELISA Kit, tnfrsf9a ELISA Kit, Tnfrsf9 ELISA Kit
- 背景
- 4-1BB (TNFRSF9/CD137)
- UniProt
- Q4W8J3
- 途径
- Cancer Immune Checkpoints
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