PTPN11 ELISA 试剂盒
PTPN11
适用: 大鼠, 人, 小鼠
pTyr542
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all PTPN11 ELISA试剂盒
- PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
- 抗原表位
- pTyr542
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适用
- 大鼠, 人, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Phospho-SHP2 (Y542) ELISA Kit. This assay semi-quantitatively measures phophorylated SHP2 (Tyr542) in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human, mouse, and rat SHP2 phosphorylated at site Tyrosine-542
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PTPN11 ELISA Kit
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Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11) ELISA Kit
PTPN11 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all PTPN11 ELISA试剂盒
- PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
- 别名
- SHP2 (PTPN11 产品)
- 别名
- BPTP3 ELISA Kit, CFC ELISA Kit, NS1 ELISA Kit, PTP-1D ELISA Kit, PTP2C ELISA Kit, SH-PTP2 ELISA Kit, SH-PTP3 ELISA Kit, SHP2 ELISA Kit, 2700084A17Rik ELISA Kit, AW536184 ELISA Kit, PTP1D ELISA Kit, SAP-2 ELISA Kit, SHP-2 ELISA Kit, Shp2 ELISA Kit, Syp ELISA Kit, SYP ELISA Kit, bptp3 ELISA Kit, cfc ELISA Kit, ns1 ELISA Kit, ptp-2 ELISA Kit, ptp2c ELISA Kit, ptpn11 ELISA Kit, ptpn11-a ELISA Kit, ptpn11-b ELISA Kit, shp-2 ELISA Kit, shp2 ELISA Kit, fa14b09 ELISA Kit, wu:fa14b09 ELISA Kit, wu:fi24f03 ELISA Kit, zgc:55388 ELISA Kit, zgc:63553 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 S homeolog ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, a ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, b ELISA Kit, PTPN11 ELISA Kit, Ptpn11 ELISA Kit, ptpn11.S ELISA Kit, ptpn11a ELISA Kit, ptpn11b ELISA Kit
- 物质类
- Viral Protein
- 背景
- SHP2 phosphorylated at Tyrosine-542
- 基因ID
- 5781
- UniProt
- Q06124
- 途径
- JAK/STAT Signaling, RTK signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effect
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