VWF ELISA 试剂盒
(5 references)-
- 抗原 See all VWF ELISA试剂盒
- VWF (Von Willebrand Factor (VWF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.13-120 ng/mL
- 最低检测浓度
- 0.13 ng/mL
- 应用范围
- ELISA
- 原理
- Human VWF ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human vWF. Other species not determined.
- 灵敏度
- 0.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling VWF ELISA Kit
- Top Product
- Discover our top product VWF ELISA Kit
-
Von Willebrand Factor (VWF) ELISA Kit
VWF 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 人 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 小鼠 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 兔 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 大鼠 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma
Von Willebrand Factor (VWF) ELISA KitVWF 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma
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- 应用备注
- Recommended Dilution for serum and plasma samples2,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
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: "The GA genotype of the -1154 G/A (rs1570360) vascular endothelial growth factor (VEGF) is protective against hypertension-related chronic kidney disease incidence." in: Molecular and cellular biochemistry, Vol. 418, Issue 1-2, pp. 159-65, (2016) (PubMed).
: "Vascular endothelial growth factor polymorphism (-460 T/C) is related to hypertension-associated chronic kidney disease." in: Clinical and experimental hypertension (New York, N.Y. : 1993), Vol. 38, Issue 5, pp. 469-75, (2016) (PubMed).
: "The GG genotype of the -152 G/A vascular endothelial growth factor (VEGF) polymorphism predisposes to hypertension-related chronic kidney disease." in: Molecular biology reports, Vol. 43, Issue 9, pp. 967-75, (2016) (PubMed).
: "The co-influence of VWD type 2B/2M mutations in the A1 domain and platelet GPIbα on the rate of cleavage to VWF by ADAMTS13." in: Thrombosis research, Vol. 136, Issue 5, pp. 987-95, (2015) (PubMed).
: "Inhibition of the NF-κB pathway by R65 ribozyme gene via adeno-associated virus serotype 9 ameliorated oxidized LDL induced human umbilical vein endothelial cell injury." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9912-21, (2015) (PubMed).
-
: "The GA genotype of the -1154 G/A (rs1570360) vascular endothelial growth factor (VEGF) is protective against hypertension-related chronic kidney disease incidence." in: Molecular and cellular biochemistry, Vol. 418, Issue 1-2, pp. 159-65, (2016) (PubMed).
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- 抗原 See all VWF ELISA试剂盒
- VWF (Von Willebrand Factor (VWF))
- 别名
- vWF (VWF 产品)
- 别名
- VWF ELISA Kit, si:ch1073-474e24.1 ELISA Kit, F8VWF ELISA Kit, VWD ELISA Kit, 6820430P06Rik ELISA Kit, AI551257 ELISA Kit, B130011O06Rik ELISA Kit, C630030D09 ELISA Kit, von Willebrand factor ELISA Kit, Von Willebrand factor ELISA Kit, VWF ELISA Kit, vwf ELISA Kit, Vwf ELISA Kit
- 背景
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Gene Names: VWF F8VWF
Protein names: von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
- 基因ID
- 7450
- UniProt
- P04275
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