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Thrombospondin 1 ELISA 试剂盒

THBS1 适用: 人 Colorimetric Sandwich ELISA 6-2000 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703490
发货至: 中国
  • 抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
    Thrombospondin 1 (THBS1)
    适用
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    6-2000 ng/mL
    最低检测浓度
    6 ng/mL
    应用范围
    ELISA
    原理
    Human Thrombospondin 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects human Thrombospondin-1. It shows less than 5 % cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4.
    灵敏度
    6 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples10 - 200 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
    Thrombospondin 1 (THBS1)
    别名
    Thrombospondin 1 (THBS1 产品)
    别名
    THBS ELISA Kit, THBS-1 ELISA Kit, TSP ELISA Kit, TSP-1 ELISA Kit, TSP1 ELISA Kit, Thbs-1 ELISA Kit, tbsp1 ELISA Kit, thrombospondin-1 ELISA Kit, thbs ELISA Kit, thbs-1 ELISA Kit, tsp ELISA Kit, tsp-1 ELISA Kit, tsp1 ELISA Kit, Tsp1 ELISA Kit, THBS1 ELISA Kit, Tsp-1 ELISA Kit, wu:fc48c03 ELISA Kit, si:dkey-11e23.1 ELISA Kit, tsp2 ELISA Kit, thrombospondin 1 ELISA Kit, thrombospondin 1 S homeolog ELISA Kit, thrombospondin 1b ELISA Kit, thrombospondin 2 L homeolog ELISA Kit, THBS1 ELISA Kit, Thbs1 ELISA Kit, thbs1.S ELISA Kit, thbs1b ELISA Kit, thbs1 ELISA Kit, thbs2.L ELISA Kit
    背景
    Gene Names: THBS1 TSP TSP1
    Protein names: Thrombospondin-1
    基因ID
    7057
    UniProt
    P07996
    途径
    Autophagy
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