JAG1 ELISA 试剂盒
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- 抗原 See all JAG1 ELISA试剂盒
- JAG1 (Jagged 1 (JAG1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.6-400 ng/mL
- 最低检测浓度
- 1.6 ng/mL
- 应用范围
- ELISA
- 原理
- Human Jagged 1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Jagged 1. Other species not yet determined.
- 灵敏度
- 1.6 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product JAG1 ELISA Kit
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Jagged 1 (JAG1) ELISA Kit
JAG1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Jagged 1 (JAG1) ELISA KitJAG1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Jagged 1 (JAG1) ELISA KitJAG1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all JAG1 ELISA试剂盒
- JAG1 (Jagged 1 (JAG1))
- 别名
- Jagged 1 (JAG1 产品)
- 别名
- AGS ELISA Kit, AHD ELISA Kit, AWS ELISA Kit, CD339 ELISA Kit, HJ1 ELISA Kit, JAGL1 ELISA Kit, ABE2 ELISA Kit, Gsfabe2 ELISA Kit, Htu ELISA Kit, Ozz ELISA Kit, Ser-1 ELISA Kit, JAG1 ELISA Kit, jag3 ELISA Kit, cb243 ELISA Kit, jag1 ELISA Kit, sb:cb243 ELISA Kit, serrateC ELISA Kit, jagged1 ELISA Kit, serrate ELISA Kit, serrate-1 ELISA Kit, x-serrate-1 ELISA Kit, jagged 1 ELISA Kit, jagged 1b ELISA Kit, jagged 1a ELISA Kit, jagged 1 L homeolog ELISA Kit, JAG1 ELISA Kit, Jag1 ELISA Kit, jag1 ELISA Kit, jag1b ELISA Kit, jag1a ELISA Kit, jag1.L ELISA Kit
- 背景
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Gene Names: JAG1 JAGL1
Protein names: Protein jagged-1 (Jagged1) (hJ1) (CD antigen CD339)
- 基因ID
- 182
- UniProt
- P78504
- 途径
- Notch Signaling, Stem Cell Maintenance
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