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BACE1 ELISA 试剂盒

BACE1 适用: 人, 小鼠 Colorimetric Sandwich ELISA 1.0-250 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2702843
发货至: 中国
  • 抗原 See all BACE1 ELISA试剂盒
    BACE1 (Beta-secretase 1 (BACE1))
    适用
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    人, 小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.0-250 ng/mL
    最低检测浓度
    1.0 ng/mL
    应用范围
    ELISA
    原理
    Human BACE-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects human BACE-1, it also can recognize mouse BACE-1.
    灵敏度
    1.0 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all BACE1 ELISA试剂盒
    BACE1 (Beta-secretase 1 (BACE1))
    别名
    BACE-1 (BACE1 产品)
    别名
    ASP2 ELISA Kit, BACE ELISA Kit, HSPC104 ELISA Kit, C76936 ELISA Kit, Bace ELISA Kit, MGC145931 ELISA Kit, BACE1 ELISA Kit, zgc:77409 ELISA Kit, beta-secretase 1 ELISA Kit, beta-site APP cleaving enzyme 1 ELISA Kit, beta-site APP-cleaving enzyme 1 ELISA Kit, BACE1 ELISA Kit, Bace1 ELISA Kit, bace1 ELISA Kit
    背景
    Gene Names: BACE1 BACE KIAA1149
    Protein names: Beta-secretase 1 (EC 3.4.23.46) (Aspartyl protease 2) (ASP2) (Asp 2) (Beta-site amyloid precursor protein cleaving enzyme 1) (Beta-site APP cleaving enzyme 1) (Memapsin-2) (Membrane-associated aspartic protease 2)
    基因ID
    23621
    UniProt
    P56817
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