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ANGPTL3 ELISA 试剂盒

ANGPTL3 适用: 小鼠 Colorimetric Sandwich ELISA 40-10.000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2702825
发货至: 中国
  • 抗原 See all ANGPTL3 ELISA试剂盒
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    40-10.000 pg/mL
    最低检测浓度
    40 pg/mL
    应用范围
    ELISA
    原理
    Mouse ANGPTL3 ELISA Kit for cell culture supernatants, EDTA treated plasma, and serum samples. Heparin and citrate are not recommended.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS1, Adiponectin, Angiopoietin-3, ANGTL-4 Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin, IFN-R1, IL-17B, IL-17B R, IL-22, MIP-1b, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGFb1, TNF RI, TNF RII, TPO, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2, VCAM-1, VEGF.
    灵敏度
    40 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples200 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    样品制备

    Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all ANGPTL3 ELISA试剂盒
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    别名
    ANGPTL3 (ANGPTL3 产品)
    别名
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    背景
    Gene Names: Angptl3
    Protein names: Angiopoietin-related protein 3 (Angiopoietin-like protein 3)
    基因ID
    30924
    UniProt
    Q9R182
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