IL-25 ELISA 试剂盒
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- 抗原 See all IL-25 (IL25) ELISA试剂盒
- IL-25 (IL25) (Interleukin 25 (IL25))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 样品类型
- Serum, Plasma, Cell Culture Cells
- 灵敏度
- 15 pg/ml
- 产品特性
- IL-25 (also known as IL-17E) is a member of the IL-17 family of proinflammatory cytokines, which includes IL-17A, IL-17B, IL-17C, IL-17F and IL-17A/F. IL-17E is found at low levels in various peripheral tissues. It is a proinflammatory cytokine that promotes airway eosinophilia in mice. IL-17E plays a role in Th2 type inflammatory diseases, such as asthma and atopic dermatitis. IL-25 induces elevated gene expression of IL-4, IL-5 and IL-13 in multiple tissues and results in T helper 2 (TH2)-type immune responses (increased serum IgE levels) and pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, increased mucus production, epithelial cell hyperplasia and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.
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- Discover our top product IL25 ELISA Kit
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Interleukin 25 (IL25) ELISA Kit
IL25 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 25 (IL25) ELISA KitIL25 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 25 (IL25) ELISA KitIL25 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 25 (IL25) ELISA KitIL25 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 25 (IL25) ELISA KitIL25 适用: 人 AA 33-177 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 25 (IL25) ELISA KitIL25 适用: 小鼠 Colorimetric Sandwich ELISA 0.03-2.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 25 (IL25) ELISA KitIL25 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
- Mouse Interleukin-25(Interleukin-17E) (IL-25(IL-17E)) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-25(IL-17E) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-25(IL-17E) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-25(IL-17E) is added to the wells and binds to the combination of capture antibody-IL-25(IL-17E) in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-25(IL-17E) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-25(IL-17)E standard dilutions and IL-25(IL-17E) sample concentration determined.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IL-25 (IL25) ELISA试剂盒
- IL-25 (IL25) (Interleukin 25 (IL25))
- 别名
- IL-25 (IL-17E) (IL25 产品)
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