Cathelicidin ELISA 试剂盒
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- 抗原 See all Cathelicidin (CAMP) ELISA试剂盒
- Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))
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适用
- 化学剂
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 1-243 pM/mL
- 最低检测浓度
- 1 pM/mL
- 应用范围
- ELISA
- 原理
- Quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.
- 样品类型
- Serum, Plasma, Saliva, Cell Culture Supernatant, Urine
- Analytical Method
- Quantitative
- 特异性
- Easy - use: All reagents and buffers for cAMP test are provided.
- 交叉反应 （详细）
- No significant cross-reactivity of similar compounds was found
- 灵敏度
- 0.61 pmol/mL
- 产品特性
- cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.
- 组件
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Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cAMP mAb: 12 ml
HRP-cAMP: 6 ml
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml): 1.5 ml
cAMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy - Top Product
- Discover our top product CAMP ELISA Kit
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Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit
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Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 大鼠 Colorimetric Competition ELISA 24.69 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 小鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.45-30 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
- The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.
- 限制
- 仅限研究用
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- 注意事项
- Do not freeze the kit.
- 储存条件
- 4 °C
- 储存方法
- The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeks at 2-8 °C.
- 有效期
- 12 months
-
- 抗原 See all Cathelicidin (CAMP) ELISA试剂盒
- Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))
- 别名
- cAMP (CAMP 产品)
- 物质类
- Chemical
- 背景
- CAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases.
- 途径
- Cellular Response to Molecule of Bacterial Origin
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