PLGF ELISA 试剂盒
(1 reference)-
- 抗原 See all PLGF (PGF) ELISA试剂盒
- PLGF (PGF) (Placenta Growth Factor (PGF))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1-400 pg/mL
- 最低检测浓度
- 1 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse PLGF-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- The sandwich ELISA antibody pair detects Mouse PLGF-2.
- 灵敏度
- 1 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PGF ELISA Kit
- Top Product
- Discover our top product PGF ELISA Kit
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Placenta Growth Factor (PGF) ELISA Kit
PGF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 大鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/M - 1000 pg/M Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 AA 21-149 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 Colorimetric Sandwich ELISA 1-400 pg/mL Cell Culture Supernatant, Plasma, Serum
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 AA 24-158 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 试剂准备
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- Bring all reagents and samples to room temperature (18 - 25 °C) before use. 2. Sample dilution: If your samples need to be diluted, 1x Assay Diluent B (Item E) should be used for dilution of cell culture supernates. Assay Diluent C (Item L) should be used for dilution of serum/plasma. Suggested dilution for normal serum/plasma: 2 fold. Please note that levels of
- 实验流程
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- Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking. 3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. The Mouse PiotaGF-2 ELISA Kit Protocol 6 9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
- 结果分析
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Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
- 实验精密度
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Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "Mesenchymal stem cells promote cardiac muscle repair via enhanced neovascularization." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 3, pp. 1219-29, (2015) (PubMed).
-
: "Mesenchymal stem cells promote cardiac muscle repair via enhanced neovascularization." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 3, pp. 1219-29, (2015) (PubMed).
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- 抗原 See all PLGF (PGF) ELISA试剂盒
- PLGF (PGF) (Placenta Growth Factor (PGF))
- 别名
- PLGF-2 (PGF 产品)
- 别名
- D12S1900 ELISA Kit, PGFL ELISA Kit, PLGF ELISA Kit, PlGF-2 ELISA Kit, SHGC-10760 ELISA Kit, AI854365 ELISA Kit, PIGF ELISA Kit, Plgf ELISA Kit, PlGF ELISA Kit, placental growth factor ELISA Kit, placenta growth factor ELISA Kit, PGF ELISA Kit, Pgf ELISA Kit, PLGF ELISA Kit
- 背景
- Placenta growth factor (PlGF)
- 基因ID
- 18654
- UniProt
- P49764
- 途径
- VEGFR1 Specific Signals
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