PCSK9 ELISA 试剂盒
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- 抗原 See all PCSK9 ELISA试剂盒
- PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
- 抗原表位
- AA 156-694
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PCSK9
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S156Q-694 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: S156Q-694 - Featured
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- Discover our top product PCSK9 ELISA Kit
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Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA Kit
PCSK9 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 人 Colorimetric Competition ELISA 0.45 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 大鼠 Colorimetric Competition ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase S8 family.
Tissue Specificity: Hepatocytes, kidney mesenchymal cells, intestinal ileum, colon epithelia and embryonic brain telencephalon neurons.
- 板类型
- Pre-coated
- 实验流程
- mouse PCSK9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PCSK9 has been precoated onto 96-well plates. Standards(NSO, S156Q-694) from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PCSK9 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse PCSK9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse PCSK9 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 194, Standard deviation: 11.25, CV(%): 5.8
- Sample 2: n=16, Mean(pg/ml): 476, Standard deviation: 20.47, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 1451, Standard deviation: 66.75, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 220, Standard deviation: 20.02, CV(%): 9.1
- Sample 2: n=24, Mean(pg/ml): 534, Standard deviation: 33.1, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 1608, Standard deviation: 120.6, CV(%): 7.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all PCSK9 ELISA试剂盒
- PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
- 别名
- PCSK9 (PCSK9 产品)
- 别名
- FH3 ELISA Kit, HCHOLA3 ELISA Kit, LDLCQ1 ELISA Kit, NARC-1 ELISA Kit, NARC1 ELISA Kit, PC9 ELISA Kit, AI415265 ELISA Kit, AI747682 ELISA Kit, Narc1 ELISA Kit, proprotein convertase subtilisin/kexin type 9 ELISA Kit, proprotein convertase subtilisin/kexin type 9 L homeolog ELISA Kit, PCSK9 ELISA Kit, pcsk9.L ELISA Kit, pcsk9 ELISA Kit, Pcsk9 ELISA Kit
- 背景
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Protein Function: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. .
Background: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Synonyms: Proprotein convertase subtilisin/kexin type 9,3.4.21.-,Neural apoptosis-regulated convertase 1,NARC-1,Proprotein convertase 9,PC9,Subtilisin/kexin-like protease PC9,Pcsk9,Narc1,
Full Gene Name: Proprotein convertase subtilisin/kexin type 9
Cellular Localisation: Cytoplasm . Secreted. Endosome . Lysosome . Cell surface . Endoplasmic reticulum . Golgi apparatus . Autocatalytic cleavage is required to transport it from the endoplasmic reticulum to the Golgi apparatus and for the secretion of the mature protein. Localizes to the endoplasmic reticulum in the absence of LDLR and co-localizes to the cell surface and to the endosomes/lysosomes in the presence of LDLR. The sorting to the cell surface and endosomes is required in order to fully promote LDLR degradation (By similarity).. - 基因ID
- 100102
- UniProt
- Q80W65
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