TNFRSF8 ELISA 试剂盒
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- 抗原 See all TNFRSF8 ELISA试剂盒
- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
- 抗原表位
- AA 19-281
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD30
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: F19-T281 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: F19-T281 - Featured
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Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA Kit
TNFRSF8 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA KitTNFRSF8 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA KitTNFRSF8 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA KitTNFRSF8 适用: 人 AA 19-379 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA KitTNFRSF8 适用: 人 Colorimetric Sandwich ELISA 20-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA KitTNFRSF8 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA KitTNFRSF8 适用: 小鼠 Colorimetric Sandwich ELISA 10-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Detected in thymus and in activated splenocytes.
- 板类型
- Pre-coated
- 实验流程
- mouse CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-T281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CD30 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD30 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 90, Standard deviation: 4.77, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 345, Standard deviation: 21.39, CV(%): 6.2
- Sample 3: n=16, Mean(pg/ml): 653, Standard deviation: 46.36, CV(%): 7.1,
- Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 6.63, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 367, Standard deviation: 27.53, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 721, Standard deviation: 60.56, CV(%): 8.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all TNFRSF8 ELISA试剂盒
- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
- 别名
- TNFRSF8 (TNFRSF8 产品)
- 别名
- CD30 ELISA Kit, D1S166E ELISA Kit, Ki-1 ELISA Kit, Cd30 ELISA Kit, Ki ELISA Kit, TNFRSF8 ELISA Kit, TNF receptor superfamily member 8 ELISA Kit, tumor necrosis factor receptor superfamily, member 8 ELISA Kit, TNFRSF8 ELISA Kit, Tnfrsf8 ELISA Kit
- 背景
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Protein Function: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B (By similarity). .
Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
Synonyms: Tumor necrosis factor receptor superfamily member 8,CD30L receptor,Lymphocyte activation antigen CD30,CD30,Tnfrsf8,
Full Gene Name: Tumor necrosis factor receptor superfamily member 8
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 21941
- UniProt
- Q60846
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