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SensoLyte® MFP Protein Phosphatase Assay Kit

EAA Fluorometric
产品编号 ABIN1882497
发货至: 中国
  • 抗原 See all EHHADH products
    EHHADH (Enoyl-CoA, Hydratase/3-Hydroxyacyl CoA Dehydrogenase (EHHADH))
    检测方法
    Fluorometric
    应用范围
    Enzyme Activity Assay (EAA)
    品牌
    SensoLyte®
    产品特性
    SensoLyte® MFP Protein Phosphatase Assay Kit uses MFP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. The kit contains:
    MFP phosphatase substrate
    Protein phosphatase inhibitor
    Assay buffer
    A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
  • 说明

    Enzymatic Activity Assay kit

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Store all kit components at -20 °C. Components B, C, D, E can be stored at room temperature for convenience.
  • Sjöström, Stenström, Eneling, Zwiller, Katz, Takemori, Bertorello: "SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 43, pp. 16922-7, (2007) (PubMed).

    Yuan, Hu, Guo, Fu, Shaw, Hu, Yao: "Phospho-regulation of HsCdc14A By Polo-like kinase 1 is essential for mitotic progression." in: The Journal of biological chemistry, Vol. 282, Issue 37, pp. 27414-23, (2007) (PubMed).

  • 抗原
    EHHADH (Enoyl-CoA, Hydratase/3-Hydroxyacyl CoA Dehydrogenase (EHHADH))
    别名
    MFP (EHHADH 产品)
    别名
    ECHD Kit, L-PBE Kit, LBFP Kit, LBP Kit, PBFE Kit, 1 Kit, Lbp Kit, MEF Kit, Mfe Kit, Mfe1 Kit, Pbe Kit, Pbfe Kit, perMFE-1 Kit, 1300002P22Rik Kit, HD Kit, MFP Kit, MFP1 Kit, zgc:77526 Kit, Ehhadh Kit, enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase Kit, enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase Kit, enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase Kit, peroxisomal bifunctional enzyme-like Kit, EHHADH Kit, ehhadh Kit, Ehhadh Kit, LOC100135519 Kit
    背景
    Protein phosphatases have received great attention as drug-screening targets. MFP is a proprietary fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The phosphatase-induced hydrolysis of MFP yields an intense fluorescent product that has excitation wavelength around 488 nm, and maximum emission around 520 nm. This characteristic makes the substrate adaptable to all the fluorescence instruments.
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