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This assay has high sensitivity and excellent specificity for detection of Estradiol (E2). No significant cross-reactivity or interference between Estradiol (E2) and analogues was observed.
Information on standard material:The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.Information on reagents:The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.Information on antibodies:The provided antibodies and their host vary in different kits.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estradiol (E2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estradiol (E2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%