TFPI ELISA 试剂盒
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- 抗原 See all TFPI ELISA试剂盒
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- 抗原表位
- AA 29-282
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFPI
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: D29-K282 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: D29-K282 - Featured
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- Discover our top product TFPI ELISA Kit
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Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA Kit
TFPI 适用: 人 Colorimetric Sandwich ELISA 0.782-50 ng/mL Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Tissue Homogenate, Urine
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Urine
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitWide Range TFPI 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitHigh Sensitivity TFPI 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Tissue Homogenate, Urine
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 马 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA KitTFPI 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Tissue Homogenate, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Contains 3 BPTI/Kunitz inhibitor domains.
Tissue Specificity: Mostly in endothelial cells.
- 板类型
- Pre-coated
- 实验流程
- human TFPI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFPI has been precoated onto 96-well plates. Standards(NSO, D29-K282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFPI amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TFPI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFPI standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 313, Standard deviation: 16.3, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 572, Standard deviation: 24.6, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 1215, Standard deviation: 47.4, CV(%): 3.9,
- Sample 1: n=24, Mean(pg/ml): 327, Standard deviation: 21.6, CV(%): 6.6
- Sample 2: n=24, Mean(pg/ml): 588, Standard deviation: 34.1, CV(%): 5.8
- Sample 3: n=24, Mean(pg/ml): 1279, Standard deviation: 79.3, CV(%): 6.2
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Tissue factor pathway inhibitor-2 inhibits the growth and invasion of hepatocellular carcinoma cells and is inactivated in human hepatocellular carcinoma." in: Oncology letters, Vol. 2, Issue 5, pp. 779-783, (2012) (PubMed).
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: "Tissue factor pathway inhibitor-2 inhibits the growth and invasion of hepatocellular carcinoma cells and is inactivated in human hepatocellular carcinoma." in: Oncology letters, Vol. 2, Issue 5, pp. 779-783, (2012) (PubMed).
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- 抗原 See all TFPI ELISA试剂盒
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- 别名
- TFPI (TFPI 产品)
- 别名
- EPI ELISA Kit, LACI ELISA Kit, TFI ELISA Kit, TFPI1 ELISA Kit, A630013F22Rik ELISA Kit, AW552122 ELISA Kit, TFPI ELISA Kit, si:dz44f8.1 ELISA Kit, tfpib ELISA Kit, wu:fb92g01 ELISA Kit, zgc:152844 ELISA Kit, zgc:158592 ELISA Kit, tissue factor pathway inhibitor ELISA Kit, tissue factor pathway inhibitor a ELISA Kit, TFPI ELISA Kit, Tfpi ELISA Kit, tfpia ELISA Kit
- 背景
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Protein Function: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
Background: TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa(Xa) and Thrombin(Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.
Synonyms: Tissue factor pathway inhibitor,TFPI,Extrinsic pathway inhibitor,EPI,Lipoprotein-associated coagulation inhibitor,LACI,TFPI,LACI, TFPI1,
Full Gene Name: Tissue factor pathway inhibitor
Cellular Localisation: Isoform Alpha: Secreted. - 基因ID
- 7035
- UniProt
- P10646
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