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TNFRSF18 ELISA 试剂盒

TNFRSF18 适用: 小鼠 AA 22-153 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1672796
发货至: 中国
  • 抗原 See all TNFRSF18 ELISA试剂盒
    TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))
    抗原表位
    AA 22-153
    适用
    • 6
    • 4
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5-4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF18/GITR
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: S22-H153
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: S22-H153
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Preferentially expressed in activated T lymphocytes.

    板类型
    Pre-coated
    实验流程
    mouse TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, S22-H153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF18 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF18 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 228, Standard deviation: 10.5, CV(%): 4.6
    • Sample 2: n=16, Mean(pg/ml): 1805, Standard deviation: 97.47, CV(%): 5.4
    • Sample 3: n=16, Mean(pg/ml): 3208, Standard deviation: 96.24, CV(%): 3,
    • Sample 1: n=24, Mean(pg/ml): 324, Standard deviation: 17.82, CV(%): 5.5
    • Sample 2: n=24, Mean(pg/ml): 2125, Standard deviation: 144.5, CV(%): 6.8
    • Sample 3: n=24, Mean(pg/ml): 3154, Standard deviation: 220.8, CV(%): 7
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Guiraud, Edwards, Squire, Babbs, Shah, Berg, Chen, Davies: "Identification of serum protein biomarkers for utrophin based DMD therapy." in: Scientific reports, Vol. 7, pp. 43697, (2018) (PubMed).

  • 抗原 See all TNFRSF18 ELISA试剂盒
    TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))
    别名
    TNFRSF18 (TNFRSF18 产品)
    别名
    GITR ELISA Kit, TNFRSF18 ELISA Kit, AITR ELISA Kit, Gitr ELISA Kit, CD357 ELISA Kit, GITR-D ELISA Kit, TNF receptor superfamily member 18 ELISA Kit, tumor necrosis factor receptor superfamily, member 18 ELISA Kit, TNFRSF18 ELISA Kit, Tnfrsf18 ELISA Kit
    背景

    Protein Function: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway (By similarity). .

    Background: Tumor necrosis factor receptor superfamily member 18(TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

    Synonyms: Tumor necrosis factor receptor superfamily member 18,Glucocorticoid-induced TNFR-related protein,CD357,Tnfrsf18,Gitr,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 18

    Cellular Localisation: Isoform A: Cell membrane, Single-pass type I membrane protein.
    基因ID
    21936
    UniProt
    O35714
    途径
    Cancer Immune Checkpoints
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