CD14 ELISA 试剂盒
(1 reference)-
- 抗原 See all CD14 ELISA试剂盒
- CD14
- 抗原表位
- AA 18-345
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD14
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: A18-P345 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: A18-P345 - Featured
- Discover our best selling CD14 ELISA Kit
- Top Product
- Discover our top product CD14 ELISA Kit
-
CD14 (CD14) ELISA Kit
CD14 适用: 人 Colorimetric Sandwich ELISA 0.234-15 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
CD14 (CD14) ELISA KitCD14 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Cell Lysate, Plasma, Serum
CD14 (CD14) ELISA KitCD14 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
CD14 (CD14) ELISA KitCD14 适用: 猴 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: Cow Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
CD14 (CD14) ELISA KitCD14 适用: 小鼠 Colorimetric Sandwich ELISA 3 ng/mL - 50 ng/mL Plasma, Serum, Urine
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse CD14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD14 has been precoated onto 96-well plates. Standards(NSO, A18-P345) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD14 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD14 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 0.98, Standard deviation: 0.038, CV(%): 3.9
- Sample 2: n=16, Mean(ng/ml): 3.11, Standard deviation: 0.162, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 6.23, Standard deviation: 0.355, CV(%): 5.7,
- Sample 1: n=24, Mean(ng/ml): 1.03, Standard deviation: 0.081, CV(%): 7.9
- Sample 2: n=24, Mean(ng/ml): 3.47, Standard deviation: 0.249, CV(%): 7.2
- Sample 3: n=24, Mean(ng/ml): 6.72, Standard deviation: 0.423, CV(%): 6.3
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Intrapulmonary expression of scavenger receptor and CD14 and their relation to local inflammatory responses to endotoxemia in mice." in: World journal of surgery, Vol. 27, Issue 1, pp. 1-9, (2003) (PubMed).
-
: "Intrapulmonary expression of scavenger receptor and CD14 and their relation to local inflammatory responses to endotoxemia in mice." in: World journal of surgery, Vol. 27, Issue 1, pp. 1-9, (2003) (PubMed).
-
- 抗原 See all CD14 ELISA试剂盒
- CD14
- 别名
- CD14 (CD14 产品)
- 别名
- CD14 molecule ELISA Kit, CD14 antigen ELISA Kit, CD14 ELISA Kit, Cd14 ELISA Kit
- 背景
-
Protein Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity). .
Background: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Synonyms: Monocyte differentiation antigen CD14,Myeloid cell-specific leucine-rich glycoprotein,CD14,Cd14,
Full Gene Name: Monocyte differentiation antigen CD14
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. - 基因ID
- 12475
- UniProt
- P10810
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
-
