SLC3A2 ELISA 试剂盒
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- 抗原 See all SLC3A2 ELISA试剂盒
- SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLC3A2 in human tissue homogenates, cell lysates and other biological fluids.
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 0.051 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2) ELISA Kit
SLC3A2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2) ELISA KitSLC3A2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - 实验流程
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The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- 有效期
- 12 months
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- 抗原 See all SLC3A2 ELISA试剂盒
- SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))
- 别名
- SLC3A2 (SLC3A2 产品)
- 别名
- 4F2 ELISA Kit, 4F2HC ELISA Kit, 4T2HC ELISA Kit, CD98 ELISA Kit, CD98HC ELISA Kit, MDU1 ELISA Kit, NACAE ELISA Kit, Mdu1 ELISA Kit, MGC53951 ELISA Kit, slc3a2 ELISA Kit, MGC53965 ELISA Kit, SLC3A2 ELISA Kit, AI314110 ELISA Kit, Cd98 ELISA Kit, Ly-10 ELISA Kit, Ly-m10 ELISA Kit, Ly10 ELISA Kit, Mgp-2hc ELISA Kit, fc65h12 ELISA Kit, wu:fc65h12 ELISA Kit, id:ibd1327 ELISA Kit, sb:cb519 ELISA Kit, wu:fe14a07 ELISA Kit, wu:fq18d12 ELISA Kit, zgc:55813 ELISA Kit, solute carrier family 3 member 2 ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog ELISA Kit, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2a ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2b ELISA Kit, SLC3A2 ELISA Kit, Slc3a2 ELISA Kit, slc3a2.L ELISA Kit, slc3a2.S ELISA Kit, slc3a2a ELISA Kit, slc3a2b ELISA Kit
- 背景
- Alternative name: SLC3A2, CD98HC, 4F2, 4F2HC, 4T2HC, MDU1, NACAE, Activators Of Dibasic And Neutral Amino Acid Transport, Antigen Identified By Monoclonal Antibodies 4F2, CD98 Heavy Chain
- 基因ID
- 6520
- UniProt
- P08195
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