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Staining Protocol: 1.Dilute the Binding buffer (10×) to work solution (1×) with distilled water. 2.Harvest cell, then wash once with cold PBS, remove the PBS from the cell pellet. 3.Wash again with cold 1× Binding buffer, remove the Binding buffer from the cell pellet. 4.Resuspend cells in cold 1× Binding buffer to a concentration of 1×10 6 cells/mL. 5.Add 100µL of cells (1×10 5 ) to each appropriate tube. 6.Add 5µL of Annexin V-FITC to appropriate tubes. 7.Gently vortex each tube and incubate for 10 minutes at room temperature in dark. 8.Add 5µL of PI solution and incubate for 5 min at room temperature in dark. 9.Wash cells once in PBS and resuspend in PBS. 10.Analyze by flow cytometry within 4 hours.