ANG ELISA 试剂盒
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- 抗原 See all ANG ELISA试剂盒
- ANG (Angiogenin (ANG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78-5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of ANG in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 12 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-human ANG antibody 2. Lyophilized human ANG standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human ANG antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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- Discover our top product ANG ELISA Kit
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Angiogenin (ANG) ELISA Kit
ANG 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Angiogenin (ANG) ELISA KitANG 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Angiogenin (ANG) ELISA KitANG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: Pig Colorimetric Competition ELISA 94 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: Pig Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Angiogenin (ANG) ELISA KitANG 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiogenin (ANG) ELISA KitANG 适用: 人 AA 25-147 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ANG polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ANG polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the ANG amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of ANG can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 30 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all ANG ELISA试剂盒
- ANG (Angiogenin (ANG))
- 别名
- Angiogenin / ANG (ANG 产品)
- 别名
- ALS9 ELISA Kit, HEL168 ELISA Kit, RNASE4 ELISA Kit, RNASE5 ELISA Kit, ANG ELISA Kit, AI385586 ELISA Kit, Ang1 ELISA Kit, Rnase5 ELISA Kit, Rnase5a ELISA Kit, ANG1 ELISA Kit, angiogenin ELISA Kit, angiogenin, ribonuclease, RNase A family, 5 ELISA Kit, ribonuclease A family member k6 ELISA Kit, ANG ELISA Kit, Ang ELISA Kit, RNASE6 ELISA Kit
- 背景
- Angiogenin (ANG) also known as ribonuclease 5, is a potent stimulator of new blood vessel formation. ANG is a member of the pancreatic ribonuclease A superfamily, and RNase activity of ANG is important for its angiogenic activity. It is expressed in the neuroaxis. Like VEGF, ANG is induced by hypoxia to elicit angiogenesis and is expressed in motor neurons. Angiogenin may function as a tRNA-specific ribonuclease that binds to actin on the surface of endothelial cells, once bound, angiogenin is endocytosed and translocated to the nucleus, thereby promoting the endothelial invasiveness necessary for blood vessel formation. Angiogenin induces vascularization of normal and malignant tissues, and abolishes protein synthesis by specifically hydrolyzing cellular tRNAs.
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