anti-马 IL21 抗体 for ELISA

Recommended IL21 Antibody (supplied by: Log in to see )

抗原
Interleukin 21 (IL21) 抗体
  • IL-21
  • Za11
  • interleukin 21
  • IL21
  • Il21
抗原表位
AA 24-152
16
15
12
9
8
8
7
6
6
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
适用
牛, 猪, 马
203
99
28
7
4
2
2
1
1
宿主

171
69
32
9
4
克隆类型
多克隆
标记
This IL21 antibody is un-conjugated
24
12
12
10
10
8
6
5
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
ELISA, Western Blotting (WB)
192
131
49
41
30
23
14
13
10
7
6
6
4
4
4
3
2
1
1
选项
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产品编号 ABIN964709
$ 583.50
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN1102475 ELISA WB Rabbit IgG AA 24-152 Log in to see Polyclonal 0

General

抗原 Interleukin 21 (IL21) 抗体
抗原表位 AA 24-152
(16), (15), (12), (9), (8), (8), (7), (6), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
适用 牛, 猪, 马
(203), (99), (28), (7), (4), (2), (2), (1), (1)
宿主
(171), (69), (32), (9), (4)
克隆类型
多克隆
标记 This IL21 antibody is un-conjugated
(24), (12), (12), (10), (10), (8), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 ELISA, Western Blotting (WB)
(192), (131), (49), (41), (30), (23), (14), (13), (10), (7), (6), (6), (4), (4), (4), (3), (2), (1), (1)
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IL21抗体详情

IL21目标详情 使用细节 贮存及处理 图像
特异性 This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
交叉反应 猪, 马
产品特性 Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common gamma-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ralpha chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. Anti-IL-21 antibody is ideal for investigators involved in Cancer and Immunology research.
过滤 Sterile filtered
免疫原 This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
Immunogen Type: RecombinantProtein
亚型 IgG
Plasmids, Primers & others

IL21目标详情

IL21抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 IL21 (IL21 Antibody 摘要)
背景 Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common γ-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Rα chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells; with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. Anti-IL-21 antibody is ideal for investigators involved in Cancer and Immunology research.
Synonyms: Interleukin-21, IL-21
基因ID 378475
UniProt Q76LU5
途径 JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

使用细节

IL21抗体详情 IL21目标详情 贮存及处理 图像 回页首
应用备注 IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kDa in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.
说明

Gene Name: IL21

限制 仅限研究用

贮存及处理

IL21抗体详情 IL21目标详情 使用细节 图像 回页首
状态 Liquid
溶解方式 Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months

图像

IL21抗体详情 IL21目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Interleukin 21 (IL21) (AA 24-152) antibody (ABIN964709) Western blot using anti-bovine IL-21 antibody shows detection of recombinant bovine ...