anti-人 IL-4 抗体 for Blocking Antibody

Recommended IL-4 Antibody (supplied by: Log in to see )

抗原
Interleukin 4 (IL4) 抗体
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
适用

391
200
51
32
24
20
19
15
14
11
11
9
9
6
6
6
6
5
2
1
宿主
大鼠
271
225
194
7
6
4
克隆类型
单克隆
标记
This IL-4 antibody is un-conjugated
85
52
33
30
22
13
13
9
9
8
7
7
7
7
7
7
5
5
4
4
4
3
2
2
2
2
1
1
应用范围
Blocking Antibody (Inhibition), Western Blotting (WB)
387
385
250
141
55
45
39
38
31
24
22
22
15
10
8
8
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
选项
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产品编号 ABIN967463
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689766 Inhibition ELISA Mouse IgG1, kappa Log in to see 8D4 2
1 ABIN2689767 Inhibition ELISA Mouse IgG1, kappa Log in to see 8D4 0
1 ABIN289347 Inhibition ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN283749 Inhibition ELISA WB Mouse IgG2a Log in to see M6110641 0
1 ABIN1002643 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal 0

General

抗原 Interleukin 4 (IL4) 抗体
适用
(391), (200), (51), (32), (24), (20), (19), (15), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (1)
宿主 大鼠
(271), (225), (194), (7), (6), (4)
克隆类型 (克隆位点)
单克隆   ( )
标记 This IL-4 antibody is un-conjugated
(85), (52), (33), (30), (22), (13), (13), (9), (9), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
应用范围 Blocking Antibody (Inhibition), Western Blotting (WB)
(387), (385), (250), (141), (55), (45), (39), (38), (31), (24), (22), (22), (15), (10), (8), (8), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5个引用可参考
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IL-4抗体详情

IL-4目标详情 使用细节 贮存及处理 References for anti-IL-4 antibody (ABIN967463) 图像
品牌 BD Pharmingen™
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Purified Recombinant Human IL-4
克隆位点 MP4-25D2
亚型 IgG1

IL-4目标详情

IL-4抗体详情 使用细节 贮存及处理 References for anti-IL-4 antibody (ABIN967463) 图像 回页首
抗原
别名 IL-4 (IL4 Antibody 摘要)
背景 The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described. This antibody is routinely tested as a blocking control for intracellular staining.
途径 JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

使用细节

IL-4抗体详情 IL-4目标详情 贮存及处理 References for anti-IL-4 antibody (ABIN967463) 图像 回页首
应用备注 1. Blocking Control for Intracellular Staining:
The unlabeled MP4-25D2 antibody can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated MP4-25D2 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled MP4-25D2 antibody (ABIN967463) for 20 minutes at 4°C, prior to staining with conjugated MP4-25D2 antibody. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

2. Neutralization:
The NA/LE™ MP4-25D2 antibody is useful for neutralization of human IL-4 bioactivity. A suitable NA/LE ™ rat IgG1 isotype control to match the MP4-25D2 antibody is the R3-34 antibody.

3. WB: The purified MP4-25D2 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested.
限制 仅限研究用

贮存及处理

IL-4抗体详情 IL-4目标详情 使用细节 References for anti-IL-4 antibody (ABIN967463) 图像 回页首
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4° C.

References for anti-IL-4 antibody (ABIN967463)

IL-4抗体详情 IL-4目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Jung, Schauer, Rieger, Wagner, Einsle, Neumann, Heusser: "Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells." in: European journal of immunology, Vol. 25, Issue 8, pp. 2413-6, 1995

Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989

图像

IL-4抗体详情 IL-4目标详情 使用细节 贮存及处理 References for anti-IL-4 antibody (ABIN967463) 回页首
厂商提供的图像
 image for anti-Interleukin 4 (IL4) antibody (ABIN967463) Expression of IL-4 by stimulated human peripheral blood mononuclear cells (PBMC). Hum...
 image for anti-Interleukin 4 (IL4) antibody (ABIN967463) The binding of PE-MP4-25D2 antibody was blocked by preincubation of the fixed/permeab...