|抗原||Interleukin 13 (IL13) 抗体|
|标记||This IL-13 antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Western Blotting (WB)
IL-13抗体详情IL-13目标详情 使用细节 贮存及处理 References for anti-IL-13 antibody (ABIN401064) 图像
|特异性||This antibody reacts to Interleukin-13.|
Species reactivity (expected):Sheep, Dolphin, Beluga, Bovine, Human, Certain monkey, Dusky titi, Macaque, Baboon, Mangabey, Canine, Llama, Camel, Galago, Horse, Rat, Mouse, Gerbil.
Species reactivity (tested):Porcine.
|纯化方法||Affinity chromatography on Protein A|
|免疫原||Full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein|
|Plasmids, Primers & others|
IL-13目标详情IL-13抗体详情 使用细节 贮存及处理 References for anti-IL-13 antibody (ABIN401064) 图像 回页首
|别名||Interleukin-13 / IL13 (IL13 Antibody 摘要)|
|背景||Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ra) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.Synonyms: IL-13, NC30|
|途径||JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport|
使用细节IL-13抗体详情 IL-13目标详情 贮存及处理 References for anti-IL-13 antibody (ABIN401064) 图像 回页首
ELISA: 1/11,000. Western Blot: 1.5 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理IL-13抗体详情 IL-13目标详情 使用细节 References for anti-IL-13 antibody (ABIN401064) 图像 回页首
|溶解方式||Restore with 100 μL of deionized water or equivalent.|
|浓度||1.0 mg/mL by UV absorbance at 280 nm|
|缓冲液||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IL-13 antibody (ABIN401064)IL-13抗体详情 IL-13目标详情 使用细节 贮存及处理 图像 回页首
Trigona, Brown, Estes: "Functional implications for signaling via the IL4R/IL13R complex on bovine cells." in: Veterinary immunology and immunopathology, Vol. 72, Issue 1-2, pp. 73-9, 2000
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