ABIN412076 产品详细信息, 供应商: Log in to see
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Supplier Product No.
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原理 A caspase-3 inhibitor
序列 Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK
产品特性 A synthetic peptide that irreversibly inhibits activity of DEVD-dependent caspases (e.g., caspase-3 and caspase-7) and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
纯度 Single spot by TLC
Chemical Name Z-DEVD-FMK, Z-DEVD-fluoromethylketone, Caspase-3 inhibitor (fluoromethylketone)
Formula C₃₀H₄₁FN₄O₁₂
Permeability Cell-permeable
Solubility DMSO (20 mM)
分子量 668.7 g/mol
应用备注 We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
The optimal doses may vary for different cells and culture conditions.
限制 仅限研究用
状态 Solid, Lyophilized
注意事项 Protect from light and moisture
储存条件 -20 °C
有效期 12 months
 image for Z-DEVD-FMK (ABIN412076) Z-DEVD-FMK
有引用在: Moon, Kim, Gao, Quan, Qin, Osorio, Rosas, Wu, Tesfaigzi, Jin: "CCN1 secretion and cleavage regulate the lung epithelial cell functions after cigarette smoke." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 307, Issue 4, pp. L326-37, 2014 (PubMed).

Kim, Amarnani, Gnanaguru, Tseng, Vavvas, DAmore: "Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms." in: Investigative ophthalmology & visual science, Vol. 55, Issue 8, pp. 4747-58, 2014 (PubMed).

Pianu, Lefort, Thuiliere, Tabourier, Bartolini: "The Aβ₁₋₄₂ peptide regulates microtubule stability independently of tau." in: Journal of cell science, Vol. 127, Issue Pt 5, pp. 1117-27, 2014 (PubMed).

Heinhuis, Koenders, van den Berg, Netea, Dinarello, Joosten: "Interleukin 32 (IL-32) contains a typical ?-helix bundle structure that resembles focal adhesion targeting region of focal adhesion kinase-1." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5733-43, 2012 (PubMed).

Du, Yamamoto, Ueda, Suzuki, Tano, Kamei: "Activated protein C rescues the retina from ischemia-induced cell death." in: Investigative ophthalmology & visual science, Vol. 52, Issue 2, pp. 987-93, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

Hara, Senga, Biswas, Hasegawa, Ito, Hyodo, Hirooka, Niwa, Goto, Hamaguchi: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

Verma, Jain, Caseltine, Rennels, Bhattacharya, Markiewski, Rawat, Neethling, Bickel, Weidanz: "TCR mimic monoclonal antibodies induce apoptosis of tumor cells via immune effector-independent mechanisms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 3265-76, 2011 (PubMed).

Vogt, Harmata, Coulombe, Bross, Blackstone: "Causes and consequences of stolon regression in a colonial hydroid." in: The Journal of experimental biology, Vol. 214, Issue Pt 19, pp. 3197-205, 2011 (PubMed).

Abdul-Ghani, Dufort, Stiles, De Repentigny, Kothary, Megeney: "Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals." in: Molecular and cellular biology, Vol. 31, Issue 1, pp. 163-78, 2010 (PubMed).

Zhong, Carney, Boyan, Schwartz: "17β-Estradiol regulates rat growth plate chondrocyte apoptosis through a mitochondrial pathway not involving nitric oxide or MAPKs." in: Endocrinology, Vol. 152, Issue 1, pp. 82-92, 2010 (PubMed).

Hirata, Osuga, Takamura, Kodama, Hirota, Koga, Yoshino, Harada, Takemura, Yano, Taketani: "Recruitment of CCR6-expressing Th17 cells by CCL 20 secreted from IL-1 beta-, TNF-alpha-, and IL-17A-stimulated endometriotic stromal cells." in: Endocrinology, Vol. 151, Issue 11, pp. 5468-76, 2010 (PubMed).

Cunningham, Giuffrida, Roberts: "Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta." in: Endocrinology, Vol. 150, Issue 12, pp. 5539-48, 2009 (PubMed).