ABIN412072 产品详细信息, 供应商: Log in to see
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Supplier Product No.
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原理 A caspase-4 inhibitor
序列 Z-Tyr-Val-Ala-Asp(OMe)-FMK
产品特性 A synthetic peptide that irreversibly inhibits activity of caspase-1. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
纯度 Single spot by TLC
Chemical Name Z-YVAD-FMK, Z-YVAD-fluoromethylketone, Caspase-1 Inhibitor (fluoromethylketone)
Formula C₃₁H₃₉FN₄O₉
Permeability Cell-permeable
Solubility DMSO (20 mM)
分子量 630.7 g/mol
应用备注 We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
The optimal doses may vary for different cells and culture conditions.
限制 仅限研究用
状态 Solid, Lyophilized
注意事项 Protect from light and moisture
储存条件 -20 °C
有效期 12 months
 image for Z-YVAD-FMK (ABIN412072) Z-YVAD-FMK
有引用在: Zhou, Shah, Yang, Zhang, Zhou, Zhao: "Virulent Mycobacterium bovis Beijing Strain Activates the NLRP7 Inflammasome in THP-1 Macrophages." in: PLoS ONE, Vol. 11, Issue 4, pp. e0152853, 2016 (PubMed).

Burm, Zuiderwijk-Sick, t Jong, van der Putten, Veth, Kondova, Bajramovic: "Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 2, pp. 678-87, 2015 (PubMed).

Kim, Amarnani, Gnanaguru, Tseng, Vavvas, DAmore: "Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms." in: Investigative ophthalmology & visual science, Vol. 55, Issue 8, pp. 4747-58, 2014 (PubMed).

Tseng, Thein, Kinnunen, Lashkari, Gregory, DAmore, Ksander: "NLRP3 inflammasome activation in retinal pigment epithelial cells by lysosomal destabilization: implications for age-related macular degeneration." in: Investigative ophthalmology & visual science, Vol. 54, Issue 1, pp. 110-20, 2013 (PubMed).

Lee, Hwang, Palaniyar, Grinstein, Philpott, Hu: "Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection." in: PLoS ONE, Vol. 7, Issue 4, pp. e35812, 2012 (PubMed).

Chang, Lin, Wu, Kuo, Wu, Shieh, Gean, Chen, Chen, Wu, Tai, Tseng: "Mechanisms underlying benzyl alcohol cytotoxicity (triamcinolone acetonide preservative) in human retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 52, Issue 7, pp. 4214-22, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

Shao: "Understanding the mechanisms of human tubal ectopic pregnancies: new evidence from knockout mouse models." in: Human reproduction (Oxford, England), Vol. 25, Issue 3, pp. 584-7, 2010 (PubMed).

Wei, Kadia, Tong, Zhang, Jia, Yang, Hu, Tambaro, Viallet, OBrien, Garcia-Manero: "The combination of a histone deacetylase inhibitor with the Bcl-2 homology domain-3 mimetic GX15-070 has synergistic antileukemia activity by activating both apoptosis and autophagy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 15, pp. 3923-32, 2010 (PubMed).