anti-Bat G Protein-Coupled Receptor 119 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended G Protein-Coupled Receptor 119 Antibody (supplied by: Log in to see )

抗原
G Protein-Coupled Receptor 119 (GPR119) 抗体
  • GPR119
  • GPCR2
  • G protein-coupled receptor 119
  • G-protein coupled receptor 119
  • GPR119
  • gpr119
  • Gpr119
抗原表位
Cytoplasmic Domain
21
19
13
5
3
2
2
1
1
适用
Bat, 人
79
36
19
3
1
宿主

70
17
克隆类型
多克隆
标记
This G Protein-Coupled Receptor 119 antibody is un-conjugated
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
44
32
31
30
26
13
13
3
2
2
2
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产品编号 ABIN1048698
$ 484.00
加上运输费 $45.00

General

抗原 G Protein-Coupled Receptor 119 (GPR119) 抗体
抗原表位 Cytoplasmic Domain
(21), (19), (13), (5), (3), (2), (2), (1), (1)
适用 Bat, 人
(79), (36), (19), (3), (1)
宿主
(70), (17)
克隆类型
多克隆
标记 This G Protein-Coupled Receptor 119 antibody is un-conjugated
(3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(44), (32), (31), (30), (26), (13), (13), (3), (2), (2), (2)
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G Protein-Coupled Receptor 119抗体详情

G Protein-Coupled Receptor 119目标详情 使用细节 贮存及处理 图像
品牌 IHC-plus™
特异性 Human GPR119. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
预测反应 Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bat (100%) Marmoset, Mouse, Hamster, Panda, Dog, Horse (95%) Rat, Elephant, Rabbit (90%) Bovine, Pig (80%).
纯化方法 Immunoaffinity purified
免疫原 Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR119. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bat (100%), Marmoset, Mouse, Hamster, Panda, Dog, Horse (95%), Rat, Elephant, Rabbit (90%), Bovine, Pig (80%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

G Protein-Coupled Receptor 119目标详情

G Protein-Coupled Receptor 119抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 GPR119 (GPR119 Antibody 摘要)
背景 Name/Gene ID: GPR119
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: GPR119, G-protein coupled receptor 2, GPCR2, HGPCR2, G protein-coupled receptor 119, G-protein coupled receptor 119
基因ID 139760
途径 Hormone Transport, Peptide Hormone Metabolism

使用细节

G Protein-Coupled Receptor 119抗体详情 G Protein-Coupled Receptor 119目标详情 贮存及处理 图像 回页首
应用备注 Approved: IHC, IHC-P (5 μg/mL)

Usage: Proteinase K treatment is required for antigen retrieval.
说明

Target Species of Antibody: Human

实验流程 The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
限制 仅限研究用

贮存及处理

G Protein-Coupled Receptor 119抗体详情 G Protein-Coupled Receptor 119目标详情 使用细节 图像 回页首
状态 Liquid
浓度 Lot specific
缓冲液 PBS, less than 0.1 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
有效期 12 months

图像

G Protein-Coupled Receptor 119抗体详情 G Protein-Coupled Receptor 119目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-G Protein-Coupled Receptor 119 (GPR119) (Cytoplasmic Domain) antibody (ABIN1048698) Anti-GPR119 antibody ABIN1048698 IHC staining of human brain, cortex. Immunohistoche...
Immunohistochemistry (IHC) image for anti-G Protein-Coupled Receptor 119 (GPR119) (Cytoplasmic Domain) antibody (ABIN1048698) Anti-GPR119 antibody ABIN1048698 IHC staining of human liver. Immunohistochemistry o...