Hba-x Protein (AA 1-142) (His tag)
HBA-X
宿主: 人
宿主: Baculovirus infected Insect Cells
Recombinant
> 85 % by SDS - PAGE
SDS
1 image
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- 抗原
- Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-142
- 宿主
- 人
- 资源
- Baculovirus infected Insect Cells
- 标记
- This Hba-x protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH
- 纯度
- > 85 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1ug of protein (determined by LAL method)
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In 50 mM Tris-HCl ( pH 7.5) containing 30 % glycerol, 0.5M NaCl, 2 mM DTT, 0.1 mM PMSF
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))
- 别名
- Hba-x
- 别名
- AI450015 Protein, Hbz Protein, hemoglobin X, alpha-like embryonic chain in Hba complex Protein, Hba-x Protein
- 背景
- RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 60.2kDa (533aa) 70-100kDa (SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_003152
- UniProt
- P23443
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